Anti-β-Catenin (Tyr-654) [γ-Catenin (Tyr-644)], Phosphospecific Antibody

Name: Anti-β-Catenin (Tyr-654) [γ-Catenin (Tyr-644)], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP4021
Price: 340.0 USD
Availability: InStock

7 Citations

Our β-Catenin (Tyr-654)[γ-Catenin (Tyr-644)] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus β-Catenin (Tyr-654)[γ-Catenin (Tyr-644)] and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-β-Catenin (CM1181) (lanes 1 & 2), anti-β-Catenin (Tyr-654) (CP4021) (lanes 3 & 4), or anti-γ-Catenin (CM1111) (lanes 5 & 6).

Immunocytochemical labeling of phosphorylated β-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-86) or β-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

An analysis of the phosphorylation of β-catenin at Y654 in Mec-1 cells transfected with the S45A mutant or an empty plasmid (mock). Where indicated, the cells were pretreated or not with ibrutinib (100 nM) for 1 h. The Western blot is representative of 3 independent experiments. Image from publication CC-BY-4.0. PMID: 38139452

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-β-Catenin (CM1181) (lanes 1 & 2), anti-β-Catenin (Tyr-654) (CP4021) (lanes 3 & 4), or anti-γ-Catenin (CM1111) (lanes 5 & 6).

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Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: CP4021

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

β-Catenin (Tyr-654)[γ-Catenin (Tyr-644)]

Scientific Background

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

CTNNB1

Molecular Weight

84/88

Immunogen

Phospho-β-Catenin (Tyr-654) synthetic peptide corresponds to amino acid residues around tyrosine 654 of human β-Catenin. This peptide sequence is highly conserved in rat, mouse, chicken, and frog β-Catenins, and has high homology to the conserved site in γ-Catenin (Tyr-644).

Immunogen Species

Human

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-β-Catenin (Tyr-86) peptide before affinity purification using phospho-β-Catenin (Tyr-654) peptide (without carrier). The antibody detects 84 and 88kDa* proteins corresponding to the molecular mass of γ-Catenin and β-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-654

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Catenin beta1, CTNNB1, catenin

UniProt ID

P35222

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Armadillo | Human
Western Blot: Armadillo | Human | Mouse
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
32225105	5ug/ml	Tomasello, L, et al. 2020. Regulative Loop between β-catenin and Protein Tyrosine Receptor Type γ in Chronic Myeloid Leukemia. International journal of molecular sciences, 2298.

Immunohistochemistry: Armadillo
PMID	Dilution	Publication
30394707	1:50	Merico, V, et al. 2019. Sertoli-immature spermatids disengagement during testis regression in the armadillo. Reproduction (Cambridge, England), 27-42.

Immunohistochemistry: Human
PMID	Dilution	Publication
26477314	not listed	Chakedis, J, et al. 2016. A novel protein isoform of the RON tyrosine kinase receptor transforms human pancreatic duct epithelial cells. Oncogene, 3249-3259.

Western Blot: Armadillo
PMID	Dilution	Publication
30394707	1:5000	Merico, V, et al. 2019. Sertoli-immature spermatids disengagement during testis regression in the armadillo. Reproduction (Cambridge, England), 27-42.

Western Blot: Human
PMID	Dilution	Publication
38139452	1:1000	Mihoub, I, et al. 2023. Identification of the Axis β-Catenin-BTK in the Dynamic Adhesion of Chronic Lymphocytic Leukemia Cells to Their Microenvironment. International Journal of Molecular Sciences, .
34252536	not listed	Boudria, R, et al. 2021. Regulatory interplay between Vav1, Syk and β-catenin occurs in lung cancer cells. Cell signalling, 110079.
27162341	not listed	Li,CC, et al. 2016. Enhancement of β-catenin activity by BIG1 plus BIG2 via Arf activation and cAMP signals. Proceedings of the National Academy of Sciences of the United States of America, 5946-5951.
26477314	not listed	Chakedis, J, et al. 2016. A novel protein isoform of the RON tyrosine kinase receptor transforms human pancreatic duct epithelial cells. Oncogene, 3249-3259.

Western Blot: Mouse
PMID	Dilution	Publication
28593951	not listed	Horejs, CM, et al. 2017. Preventing tissue fibrosis by local biomaterials interfacing of specific cryptic extracellular matrix information. Nature communications, 15509.

Additional Publications: Unspecified
PMID	Publication
39063166	Rebuzzini, P., et al. 2024. BPA Exposure Affects Mouse Gastruloids Axial Elongation by Perturbing the Wnt/β-Catenin Pathway. International Journal of Molecular Sciences, .

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Anti-β-Catenin (Tyr-654) [γ-Catenin (Tyr-644)], Phosphospecific Antibody

SKU: CP4021

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-β-Catenin (Tyr-654) [γ-Catenin (Tyr-644)], Phosphospecific Antibody

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