Cell Viability, Metabolism Assays and Biochemicals

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Green Fluorescent FAM-Leu-DAP Serine Protease Assay KitGreen Fluorescent FAM-Leu-DAP Serine Protease Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-Leu-DAP Serine Protease Assay Kit
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Green Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay KitGreen Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay Kit
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Green Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay KitGreen Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay Kit
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Figure 1. Jurkat cells, grown in suspension, were incubated with 1 µM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (Cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (Cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.
ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-9 (LEHD) Assay Kit
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Red Fluorescent SR101-Leu-CMK Serine Protease Assay KitRed Fluorescent SR101-Leu-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Red Fluorescent SR101-Leu-CMK Serine Protease Assay Kit
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Red Fluorescent SR101-Phe-CMK Serine Protease Assay KitRed Fluorescent SR101-Phe-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Red Fluorescent SR101-Phe-CMK Serine Protease Assay Kit
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Figure 1. Jurkat cells were untreated (A.), or were exposed to staurosporine to induce apoptosis and increase serine protease activity (B.). During the final hour of treatment, samples were stained with FAM-Leu-CMK (Cat. 949/950) for 1 hour at 37°C, washed, stained with PI for 15 minutes, and then analyzed using a flow cytometer. Staurosporine increased median fluorescence intensity for both FAM-Leu-CMK (BL1-H, x-axis) and PI (YL1-H, y-axis). Data courtesy of Dr. K. Strandberg (ICT 231:80-81).
ImmunoChemistry Technologies Green Fluorescent FAM-Leu-CMK Serine Protease Assay Kit
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Figure 1. Jurkat cells were treated with staurosporine for 1, 2, 3, and 4 hours to induce apoptosis and increase serine protease activity (green), or were untreated (gray). Samples were stained with FAM-Phe-CMK (Kit 945/946) for 1 hour at 37°C, washed, and then analyzed using a flow cytometer. The amount of serine protease activity detected positively correlated to the duration of the exposure period. Data courtesy of Dr. Kristi Strandberg (ICT 231:75-79).Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis and increase serine protease activity (upper), or left untreated (lower), then stained with FAM-Phe-CMK (Cat. 945/946), 660-VAD-FMK (Cat. 9120), and Hoechst 33342. Imaged using EGFP (Ex 470/30, Em 530/50), Cy5 (Ex 620/60, Em 700/75), and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Staurosporine increased the intracellular levels of both serine proteases and caspases. Data courtesy of Dr. K. Strandberg (ICT 231:73).
ImmunoChemistry Technologies Green Fluorescent FAM-Phe-CMK Serine Protease Assay Kit
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Flow cytometry analysis of the caspase-10 activity in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit
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Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay KitGreen Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit
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Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine for 2 hours to induce apoptosis. Cells were then stained with JC-1 dye. Normal healthy cells (two cells, upper and lower left) concentrate JC-1 in mitochondria and fluoresce bright orange. Apoptotic cells (three cells, right), with mitochondria of various stages of permeability, exhibit a reduced orange fluorescence and increased green fluorescence, as JC-1 disperses through the cells. (Dr. Brian Lee, ICT).Figure 2. Jurkat and HL-60 cells were exposed to DMSO (negative control) (dark orange) or 50 μM CCCP depolarizing agent (light orange) and then incubated with JC-1 for 20 minutes at 37°C, and washed. Aliquots were analyzed in triplicate in a fluorescence plate reader set at 488 nm excitation and 590 nm emission filter settings. Healthy cells exhibited a high level of orange fluorescence; the CCCP-depolarized cells had reduced orange fluorescence as the dye dispersed. (Ms. Tracy Hanson, ICT)
ImmunoChemistry Technologies JC-1 Fluorescent Mitochondrial Membrane Depolarization Assay
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Figure 1. Activity of caspase-8 (using FAM-LETD-FMK) in AGS cells after 24 h incubation with Les-4367 (1 μM) and combined with anti-HER2 antibodies. From Figure 4 of A. Gornowicz et al Multi-Targeting Anticancer Activity of a New 4-Thiazolidinone Derivative with Anti-HER2 Antibodies in Human AGS Gastric Cancer Cells, Int J Mol Sci (2023) 24, 6791. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.Flow cytometry analysis of the caspase-8 activity (Assay kit, Cat. no. 99) in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-8 (LETD) Assay Kit
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Figure 1. Caspase activities in the microglia of white matter injury mice using our FAM-FLICA® kits. Only caspase 1 was significantly upregulated. Figure 1 (d) of L. He et al (2022) miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury, Hindaw Journal of Immunology Research 2022, 1642896. https://doi.org/10.1155/2022/1642896. Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 Liufang He et al.
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-6 (VEID) Assay Kit
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