Cell Viability, Metabolism Assays and Biochemicals

110 products

Showing 97 - 110 of 110 products
View
Green Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay KitGreen Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay Kit
From $212.20
Choose options
Green Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay KitGreen Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay Kit
From $212.20
Choose options
Figure 1. Jurkat cells, grown in suspension, were incubated with 1 µM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.
ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-9 (LEHD) Assay Kit
From $222.50
Choose options
Red Fluorescent SR101-Leu-CMK Serine Protease Assay KitRed Fluorescent SR101-Leu-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Red Fluorescent SR101-Leu-CMK Serine Protease Assay Kit
From $212.20
Choose options
Red Fluorescent SR101-Phe-CMK Serine Protease Assay KitRed Fluorescent SR101-Phe-CMK Serine Protease Assay Kit
ImmunoChemistry Technologies Red Fluorescent SR101-Phe-CMK Serine Protease Assay Kit
From $212.20
Choose options
Figure 1. Jurkat cells were untreated (A.), or were exposed to staurosporine to induce apoptosis and increase serine protease activity (B.). During the final hour of treatment, samples were stained with FAM-Leu-CMK (Cat. 949/950) for 1 hour at 37°C, washed, stained with PI for 15 minutes, and then analyzed using a flow cytometer. Staurosporine increased median fluorescence intensity for both FAM-Leu-CMK (BL1-H, x-axis) and PI (YL1-H, y-axis). Data courtesy of Dr. K. Strandberg (ICT 231:80-81).
ImmunoChemistry Technologies Green Fluorescent FAM-Leu-CMK Serine Protease Assay Kit
From $212.20
Choose options
Figure 1. Jurkat cells were treated with staurosporine for 1, 2, 3, and 4 hours to induce apoptosis and increase serine protease activity (green), or were untreated (gray). Samples were stained with FAM-Phe-CMK (Kit 945/946) for 1 hour at 37°C, washed, and then analyzed using a flow cytometer. The amount of serine protease activity detected positively correlated to the duration of the exposure period. Data courtesy of Dr. Kristi Strandberg (ICT 231:75-79).Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis and increase serine protease activity (upper), or left untreated (lower), then stained with FAM-Phe-CMK (Cat. 945/946), 660-VAD-FMK (cat. 9120), and Hoechst 33342. Imaged using EGFP (Ex 470/30, Em 530/50), Cy5 (Ex 620/60, Em 700/75), and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Staurosporine increased the intracellular levels of both serine proteases and caspases. Data courtesy of Dr. K. Strandberg (ICT 231:73).
ImmunoChemistry Technologies Green Fluorescent FAM-Phe-CMK Serine Protease Assay Kit
From $212.20
Choose options
Flow cytometry analysis of the caspase-10 activity in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-10 (AEVD) Assay Kit
From $222.50
Choose options
Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay KitGreen Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-2 (VDVAD) Assay Kit
From $222.50
Choose options
Figure 1. HL-60 cells were treated with camptothecin (cat. 6210), followed by SR-VAD-FMK (cat. 916/917) and green FAM-FLISP serine protease inhibitor FFCK (cat. 946), and then analyzed on a scanning laser cytometer. Active caspases stain red (X-axis) and active serine proteases stain green (Y-axis). Co-localization of caspase and serine protease activity is evident in B, C, D, and G. Data courtesy of Dr. J. Grabarek, Brander Cancer Center, NY, and Pomeranian School of Medicine, Szczecin, Poland.Figure 2. Paramecium were starved for 12-24 hours in 5 mM KCl to induce autogamy. SR-VAD-FMK (cat. 916/917), was added to the live Paramecium for 30 minutes in the dark. Cells were then washed and Hoechst 33342 was added. Cells were incubated in the dark for 10 minutes, washed, and applied to coverslips. Images were captured using a Nikon TE-300 Epi-fluorescent microscope with a SPOT-RT digital camera and merged using SPOT software. Data courtesy of Dr. W. E. Bell, Virginia Military Institute.
ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Poly (active) Caspase (VAD) Assay Kit
From $222.50
Choose options
Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine for 2 hours to induce apoptosis. Cells were then stained with JC-1 dye. Normal healthy cells (two cells, upper and lower left) concentrate JC-1 in mitochondria and fluoresce bright orange. Apoptotic cells (three cells, right), with mitochondria of various stages of permeability, exhibit a reduced orange fluorescence and increased green fluorescence, as JC-1 disperses through the cells. (Dr. Brian Lee, ICT).Figure 2. Jurkat and HL-60 cells were exposed to DMSO (negative control) (dark orange) or 50 μM CCCP depolarizing agent (light orange) and then incubated with JC-1 for 20 minutes at 37°C, and washed. Aliquots were analyzed in triplicate in a fluorescence plate reader set at 488 nm excitation and 590 nm emission filter settings. Healthy cells exhibited a high level of orange fluorescence; the CCCP-depolarized cells had reduced orange fluorescence as the dye dispersed. (Ms. Tracy Hanson, ICT)
ImmunoChemistry Technologies JC-1 Fluorescent Mitochondrial Membrane Depolarization Assay
From $232.80
Choose options
Figure 1. Activity of caspase-8 (using FAM-LETD-FMK) in AGS cells after 24 h incubation with Les-4367 (1 μM) and combined with anti-HER2 antibodies. From Figure 4 of A. Gornowicz et al Multi-Targeting Anticancer Activity of a New 4-Thiazolidinone Derivative with Anti-HER2 Antibodies in Human AGS Gastric Cancer Cells, Int J Mol Sci (2023) 24, 6791. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.Flow cytometry analysis of the caspase-8 activity (Assay kit, Cat. no. 99) in the MCF-7 (A,B) and MDA-MB-231 (C,D) breast cancer cells after 24 h of incubation with Les-6287 (1.0 μM and 1.5 μM), doxorubicin (1.0 μM), and DMSO. Image from publication, CC-BY-4.0, PMID: 39199694.
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-8 (LETD) Assay Kit
From $222.70
Choose options
Figure 1. Caspase activities in the microglia of white matter injury mice using our FAM-FLICA® kits. Only caspase 1 was significantly upregulated. Figure 1 (d) of L. He et al (2022) miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury, Hindaw Journal of Immunology Research 2022, 1642896. https://doi.org/10.1155/2022/1642896. Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 Liufang He et al.
ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-6 (VEID) Assay Kit
From $222.70
Choose options
Figure 1. Fluorescent Microscopy Analysis of THP-1 Suspension Cells THP-1 cells were stained with Poly (active) Caspase FAM FLICA® Inhibitor Reagent (green), and Hoechst 33342 (Blue). Cells were incubated with staurosporine to induce apoptosis. Two photos were taken and superimposed. Only one cell of the three cells is apoptotic (middle) – it is stained positive for caspase activity with FAM-VAD-FMK. Data courtesy of Dr. Brian W. Lee.Figure 2. Caspase Activity in Jurkat Cells Jurkat cells (T lymphocytes) were labeled with our Green Fluorescent Poly (active) Caspase FAM FLICA® Inhibitor Reagent (FAM-VAD-FMK) (catalog 92) and viewed under a fluorescence microscope. The grey DIC image (B) reveals five cells in the field of view, but only four of them fluoresce green (A). Four out of five cells are apoptotic and have active caspases present (green). Data courtesy of Dr. Brian W. Lee, ICT.
ImmunoChemistry Technologies Green Fluorescent Poly (active) Caspase FAM FLICA® Assay Kit (FAM-VAD-FMK)
From $222.70
Choose options

Recently Viewed