aCella-TOX Bioluminescent Cytotoxicity Assay

A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.

5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.
5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2 . The ADCC reaction was further incubated for 3.5 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay. 3.5hour timepoint has been optimized with the 10:1 E:T ratio.
The graph shows an antibody titration of Rituximab from0.000977 to 1ug/ml incubated with 5% baby rabbit complement and 5000 Daudi target cells for 30 minutes at 37oC
5000 Daudi cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % ADCC was measured using the aCella-TOX assay.Log (EC50) value for E:T 25:1 was -2.344 and for E:T 10:1 was -2.213
A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log (EC50) value for aCella-TOX was -2.23, and that for 51Cr was -2.087 for Donor A.
A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log(EC50) value for aCella-TOX was -2.612, and that for51Cr was -2.77 for Donor B.
5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.
5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.
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Data Sheet Safety Data Sheet

SKU: CLATOX100-4

Size: 1000 Tests
Price:
$1,793.70
Ships: 1-2 business days
aCella-TOX Bioluminescence Cytotoxicity Assay Protocol
CLATOX100-3P: 500 Tests (with 5 Lumi Plates + 5 TC Plates)
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C)
  • 7. 5 Luminescence Plates + 5 Tissue Culture Plates (Catalog# CLATOX100-3P)

    • CLATOX100-3: 500 Tests
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C)

    • CLATOX100-4: 1000 Tests
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 2 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 2 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 2 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 2 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 2 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 2 (Storage: 2-8°C)

    • CLATOX100-3L: 500 Tests (with 5 Lumi Plates)
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C)
  • 7. 5 Luminescence Plates (Catalog# CLATOX100-3L)

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