Cell Viability Assays for Neuroscience Research

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Figure 1. Fluorescence spectraFigure 2. Jurkat cells were exposed to 3% formaldehyde for 30 minutes. Following the formaldhehyde treatment, cells were stained with Green Live/ Dead stain and then imaged with a Nikon E800 microscope (DIC overlay shown). Cells with compromised membranes stained green, while cells with intact membranes excluded the Green Live/Dead stain and remained unstained. Data courtesy of Mrs. Tracy Murphy, ICT.
ImmunoChemistry Technologies Green Live/Dead Stain
$108.30
DAPI Nuclear StainImmunolabeling of an organ cultured human hair follicle treated with 1.25ug/ml of ICP5249 for 5 days labeling light chain 3 I/II (green) and DAPI (Cat. 6244, blue) was used for nuclear staining. Image from publication. CC-BY-4.0 PMID: 39155393
ImmunoChemistry Technologies DAPI Nuclear Stain
$126.85
Figure 1. Jurkat cells were untreated (live) (A) or treated with 90% ethanol (dead) (B). Cells were pelleted by centrifugation (200 x g for 5 minutes) and resuspended in PBS. Cells were then stained with 7-AAD for 10 minutes on ice, and analyzed using an Accuri C6 flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B) (ICT 226:30-31).Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis, then dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red dye 7-AAD to detect necrosis. Early apoptotic cells fluoresce green with FAM-FLICA. Dually stained green and red fluorescing cells are in mid to late stage apoptosis (active caspases and compromised cell membranes). Necrotic cells fluoresce red. (ICT 196:70).
ImmunoChemistry Technologies 7-AAD Red Fluorescent Live/Dead Stain
$181.10
Figure 1. Staining of Healthy Jurkat Cells Healthy Jurkat cells were stained with CFSE or left unstained and then analyzed by flow cytometry using an Accuri C6 flow cytometer equipped with a FL1 99% attenuation filter. Unstained Jurkat cells (A), stained Jurkat cells (B), and the corresponding overlay (C) are shown.Figure 2. Identification of CFSE-stained Cells. K562 target cells were stained green with CFSE and then subjected to effector cells. Green stained target cells are easily identifiable when analyzed by FL1 vs. SSC on a flow cytometer. Target cells stained green with CFSE move to the right along the X-axis (FL1) of the plot (right) compared with unstained effector cells. This plot becomes particularly important when gating on target cells that are the same size as effector cells.
ImmunoChemistry Technologies CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
$127.15
Figure 1. Normal Jurkat cells stained with Acridine Orange (AO) show orange lysosomal staining. Cells were stained with 5 µM AO in PBS for 60 minutes at 37°C. Photomicrographs were taken using a Nikon Eclipse E800 photomicroscope using a 460-500 nm excitation filter and a 505-560 nm emission / barrier filter set at 300X. Photo B shows the corresponding DIC image of the cells in A (AO appears faintly). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Institute, New York, NY).Figure 2. MCF-7 cells were treated with camptothecin to induce apoptosis (Cat. 6210), stained with AO in PBS, washed, and then photographed with an epifluorescence microscope at 40X using either a blue light excitation (492 nm) with a 540- 550 nm emission filter (A, lysosomes appear yellowish green), or green light excitation (540 nm) with a long pass >640 nm barrier filter (B, lysosomes appear red). Data courtesy of Dr. Zbigniew Darzynkiewicz (Brander Cancer Research Center Institute, New York, NY).
ImmunoChemistry Technologies Acridine Orange Fluorescent Staining Solution
$23.90
Figure 1. A pool of Jurkat cells was spiked with FAM-YVAD-FMK (Cat. 9146) and divided into separate treatment groups. Starting with 24 hour samples and working backwards, 10 µM Nigericin was added to cells and the samples were incubated at 37°C throughout the induction process. The cells were washed and analyzed on a flow cytometer. The level of caspase-1 activity directly correlated to the period of exposure to Nigericin Data. courtesy of Mrs. Tracy Murphy, ICT (220:78).
ImmunoChemistry Technologies Nigericin
$47.65
ImmunoChemistry Technologies Tumor Necrosis Factor beta
$378.95
ImmunoChemistry Technologies Tumor Necrosis Factor alpha
$699
Staurosporine
ImmunoChemistry Technologies Staurosporine
$96.30
Camptothecin
ImmunoChemistry Technologies Camptothecin
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Figure 1. Jurkat cells were treated with staurosporine (Positive, Induced, right histogram). Negative control cells were spiked with a DMSO vehicle control (Negative, Non-induced, left histogram). Cells were stained with SR-VAD-OPH for 1 hour, washed twice, and read on a flow cytometer. Only 8.0% of control cells displayed caspase activity, whereas staurosporine induced caspase activity in 90.3% of the experimental cells. This is a ratio of 11:1. Data courtesy of Ms. Tracy Hanson, ICT 092713.Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis, then labeled with SR-VAD-OPH for 60 minutes. Cells were washed twice and wet-mount slides were prepared. A grey DIC image (left) was taken, and red fluorescence (right) was detected using a band pass filter (excitation at 565 nm, emission at 590-600 nm). SR-VAD-OPH reveals complete caspase activation in the form of red fluorescence in all cells. Data courtesy of Ms. Tracy Hanson, ICT (ICT111809; SR_OPH_111_17_18).
ImmunoChemistry Technologies Red Fluorescent SR-VAD-OPH in vitro Poly Caspase Apoptosis Detection Reagent
$584.60
Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, and then labeled with FAM-DEVD-OPH (Cat. 6356). Wet-mount slides were prepared and green fluorescence (right) was detected using a band pass filter (excitation at 488 nm, emission at 520 nm). FAM-DEVD-OPH reveals complete caspase-3/7 activation in the form of green fluorescence in all cells. The corresponding DIC image is shown (left). Data courtesy of Ms. Tracy Hanson, ICT (ICT111709; FAM-DEVD-OPH_in_59_60).
ImmunoChemistry Technologies Green Fluorescent FAM-DEVD-OPH in vitro caspase-3/7 Apoptosis Detection Reagent
$584.60
Figure 1. Jurkat cells were treated with DMSO (left) or staurosporine (right), labeled with FAM-VAD-OPH II, washed, and stained with PI. Four cell populations are seen: unstained live cells do not fluoresce (lower left); early apoptotic cells fluoresce green with FAM- VAD-OPH II (lower right); late apoptotic cells (upper right) fluoresce green (active caspases) and red (permeabilized cell membrane); and necrotic cells fluoresce red (upper left). Data of Dr. M. Olin, University of Minnesota.
ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH II in vitro poly Caspase Apoptosis Detection Reagent
$584.60
Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, then stained with FAM- VAD-OPH I (Cat. 6354) or FAM-VAD-FMK FLICA® (Cat. 92), washed and then read on a flow cytometer. Results with the OPH inhibitor (grey solid) are consistent with those achieved using the FMK inhibitor (green outline). FAM-VAD-OPH inhibitors mirror FAM-VAD-FMK analogs for apoptosis detection, and they may be less toxic and more stable. Data courtesy of Dr. M. Olin, University of Minnesota (ICT-202).
ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH I in vitro poly Caspase Apoptosis Detection Reagent
$584.60
Figure 1. Signals leading to activation of the NLRP3 inflammasome.Figure 2. THP-1 cells were treated with PMA (5 ng/mL) followed by LPS (100 ng/mL for 1 hour, lower row of images), or were untreated (upper row of images), and were then immediately stained with 660-YVAD-FMK for 1 hour at 37°C (concurrent with treatment). Cells were then stained with Hoechst 33342 and imaged. Intracellular caspase-1 activity was detected using Cy5 (Ex 620/60, Em 700/75) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Data courtesy of Mrs. Tracy Murphy (ICT 230:32).
ImmunoChemistry Technologies Pyroptosis/Caspase-1 Assay - Far Red Fluorescent
$392.50
Figure 1. Jurkat cells were stained with DAF-2DA dye (Kit 9155), washed, and then treated with DMSO control (left histogram) or 1 mM DEA NONOate, a nitric oxide donor (middle histogram). Cells were read on the FL1 channel of a flow cytometer. The median fluorescence intensity (MFI) of DEA NONOate treated cells was 3.5-fold higher than for the control cells. Data also overlaid in a single plot (right, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 227:76.Figure 2. Jurkat suspension cells were stained with DAF-2DA dye, washed, and then treated with DMSO control (Panels A-C) or DEA NONOate (Panels D-F), a nitric oxide donor. Cells treated with DEA NONOate showed an increased level of green fluorescence relative to the untreated cells (Panels A v and D). Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 228:57-58).
ImmunoChemistry Technologies Nitric Oxide Synthase Assay
$227.90
Figure 1. Live cells with green fluorescence (right side of histogram) can be distinguished from dead cells (unstained, left side of histogram). Forward and side scatter graphs are also shown. Jurkat cells were stained with 1 µM Calcein AM and analyzed in a flow cytometer with a FL-1 99% attenuation filter. 93.0% of the live cells stained positive with Calcein AM (A, right), while 0.0% of ethanol-killed cells stained positive (B, right). Data courtesy of Dr. Kristi Strandberg, ICT 226:87-92.Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of ethanol-treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.
ImmunoChemistry Technologies Advanced Calcein AM Cell Viability Kit
$305.60
Figure 1. Live cells exhibiting green fluorescence (right of histogram) are easily distinguished from dead cells (unstained, left of histogram). Jurkat cells were either mock-treated (A) or killed by exposure to 90% ethanol (B). Cells were stained with Calcein AM, then analyzed in a flow cytometer with a FL-1 99% attenuation filter. Most (93.0%) live cells stained positive with Calcein AM (A, right), while 0.0% of the dead cells stained positive (B, right). Data courtesy of Dr. K. Strandberg.Figure 2. Mock-treated Jurkat suspension cells were stained with Calcein AM to detect live cells. The majority of the cells imaged were considered to be live and healthy. Panel A reveals green fluorescence-stained live cells. Panel B shows a corresponding differential interference contrast (DIC) image, which reveals cell morphology. Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 226:95).
ImmunoChemistry Technologies Basic Calcein AM Cell Viability Kit
$247.35
Figure 1. Jurkat cells were stained with the Intracellular Total ROS Green dye (Cat. 9144), then treated with a negative control (left) or 100 µM tert-Butyl hydroperoxide (ROS-inducing agent) (middle). The median fluorescence intensity (MFI) of stained negative control cells was 11,875 in FL1-A (Negative) and 419,067 for ROS-induced cells (Positive). The data are also shown in overlay (black: Negative, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 224:76.
ImmunoChemistry Technologies Intracellular Total ROS Activity Assay
$359.75
Figure 1. Typical standard curves. A new curve must be generated each time the assay is run.Figure 2. Typical nitrite linearity curve
ImmunoChemistry Technologies Nitric Oxide Colorimetric Detection Kit
$357.70
Figure 1. Structure of GlutathioneFigure 2. Glutathione Reactions
ImmunoChemistry Technologies Glutathione Colorimetric Detection Kit
$525
Glutathione Fluorescent Detection KitGlutathione Fluorescent Detection Kit
ImmunoChemistry Technologies Glutathione Fluorescent Detection Kit
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Figure 1. Typical standard curve. A new curve must be generated each time the assay is run.Figure 2. Typical linearity curve
ImmunoChemistry Technologies Hydrogen Peroxide Colorimetric Detection Kit
$465.05
Figure 1. Typical standard curve. A new curve must be generated each time the assay is run.Figure 2. Typical linearity curve.
ImmunoChemistry Technologies Hydrogen Peroxide Fluorescent Detection Kit
$465.05

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