Fluorescent Hypochlorite Detection Kit

Two cell-permeable dyes hydroxyphenyl fluorescein (HPF) and aminophenyl fluorescein (APF) detect hypochlorite production in cells. Hypochlorite production is observed by increased fluorescence of APF loaded cells with no increase in HPF loaded cells

Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).
Figure 2. Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488 nm emission: 505-550 nm barrier filters. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.
Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).
Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).
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Data Sheet

SKU: FLOCL100-2

Size: 100 Tests
Price:
$533.50
Ships: 1-2 business days
Fluorescent Hypochlorite Detection Kit Protocol
  • Part# 4012: HPF Dye, 1 Vial (2-8C)
  • Part# 4011: APF Dye, 1 Vial (2-8C)

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