Fluoro NAD/NADH Detection Kit by Cell Technology

Name: Fluoro NAD/NADH Detection Kit by Cell Technology
Brand: ImmunoChemistry Technologies
SKU: FLNADH100-3
Price: 2988.45 USD
Availability: InStock

The kit uses a fluorescent readout to specifically detect NAD/NADH, with no reactivity with NADP/NADPH. It is suitable for use with cells and tissue from a range of sources such as animals, plants, bacteria and fungi.

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.

Figure 3. The assay reacts with NAD and NADH. There is no reactivity with NADPH.

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

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Data Sheet Safety Data Sheet

SKU: FLNADH100-3

Size: 500 Tests

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Price:

$2,988.45

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Ships: 1-2 business days

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Scientific Background

The role of pyridine nucleotides (NAD/NADH) metabolism in health is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation. These enzymes are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling, which suggests that either the enzymes or NAD could be an important therapeutical target.

The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH.

The kit is suitable for applications such as:
- Detection of NAD/NADH activity in cells or tissue extracts.
- Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.
- NAD/NADH detection in bacterial, fungal and plant cells.

Excitation / Emission

530-570nm/590-600nm

Method of Analysis

Fluorescence plate reader

Usage Statement

For research use only. Not for use in diagnostic procedures.

Country of Origin

United States

Storage

Store contents as labeled

Shipping

Ships overnight (domestic), International Priority Shipping

Fluoro NAD/NADH Detection Kit Protocol

FLNADH100-2: 100 Tests

Part# 6021: Enzyme Mix x 1 (-20C)

Part# 4018: NADH Detection Reagent x 1 (-20C)

Part# 7013: NADH Standard x 1 (-20C)

Part# 3044: NAD/NADH Reaction Buffer x 1 (2-8 C)

Part# 3045: NAD/NADH Lysis Buffer x 1 (2-8 C)

Part# 3046: NAD Extraction Buffer x 1 (2-8 C)

Part# 3047: NADH Extraction Buffer x 1 (2-8 C)

Part# 3051: NADH Standard Diluent x 1 (2-8 C)

FLNADH100-3: 500 Tests

Part# 6021: Enzyme Mix x 5 (-20C)

Part# 4018: NADH Detection Reagent x 5 (-20C)

Part# 7013: NADH Standard x 5 (-20C)

Part# 3044: NAD/NADH Reaction Buffer x 5 (2-8 C)

Part# 3045: NAD/NADH Lysis Buffer x 5 (2-8 C)

Part# 3046: NAD Extraction Buffer x 5 (2-8 C)

Part# 3047: NADH Extraction Buffer x 5 (2-8 C)

Part# 3051: NADH Standard Diluent x 5 (2-8 C)

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Fluoro NAD/NADH Detection Kit by Cell Technology

SKU: FLNADH100-3

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