Endogenous Peroxidase Block
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears.
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears. Peroxidase is most commonly found in red blood cells, myoglobin in muscle cells, cytochrome in granulocytes, and monocytes, liver and kidney. The presence of endogenous peroxidase activity gives false results when using peroxidase based Immunohistochemistry technique. For frozen tissue sections eg. Peripheral blood, bone marrow, fixation should be done with acetone/methanol.
Ready to Use Blocking Solution
Cell Specially Blood, Frozen Tissues, Necrotic Tissues, FFPE Sections
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For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
- Deparaffinizate the tissue as described in your protocol.
- For paraffin sections add few drops of endogenous peroxidase blocking solution for 10 minutes at room temperature. For frozen sections use Endogenous Peroxidase blocking solution (1:10 diluted in methanol) . Rinse slide with distilled water 3X.
- Proceed with the protocol.