Donkey Serum
This serum is used as blocking agents at 5% in buffer with all Immunoassays, to reduce non-specific background and conserved sequence. This serum can also be used as a negative control in immunoassays with proper dilution.
Donkey Serum
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Ships: 1-2 business days
For Immunohistochemistry (IHC) and Immunocytochemistry (ICC):
- If tissues are used, deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol).
- Wash 2-3 with distilled or deionized water. 3. Incubate sections/cell smear in Endoblocker (#1) for 5-10 minutes at room temperature or 37°C. Note: If antigen retriever is required it can be applied after this stage.
- Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC cat # AR-6561) 3-5X.
- Incubate sections/cell smear in Protein blocking solution (diluted animal serum), for 10 minutes at RT or 37ºC.
- Wash slide with PBS 1X.
- Follow instructions for IHC/ICC.
Product Specific References
| PMID | Publication |
| 39929589 | Hirabayashi, S, et al. 2025. CCN1 derived from vascular endothelial cells impairs cognitive function in Alzheimer's disease model mice. Journal of Pharmacological Sciences, 146-155. |
| 39812695 | Ohe, Y, et al. 2025. Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae. Journal of Comparative Physiology, . |