Fluoro-Jade C (FJC) Staining Kit

Name: Fluoro-Jade C (FJC) Staining Kit
Brand: Biosensis
SKU: TR-100-FJ
Price: 517.0 USD
Availability: InStock

The Fluoro-Jade C Ready-to-Dilute (RTD) Staining Kit provides an easy to use assortment of Fluoro-Jade C, DAPI, sodium hydroxide and potassium permanganate in liquid form. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.

Fluoro-Jade C (FJC) staining (green) of degenerating neurons in the CA3 region of the hippocampus in old diabetic rats. Blue: DAPI nuclear stain. Picture courtesy of Dr. Wang and colleagues. Results of this study were published in Wang S. et al. (2020), Geroscience, PMID: 32696219.

Double exposure using combined blue and ultraviolet epi-fluorescent illumination of the superficial layers of the cingulated rat cortex exposed to kainic acid. Layer I contains conspicuous Fluoro-Jade C positive degenerating axon terminals. Layer II contains densely packed DAPI-positive viable granule cells. Layer III contains a mixture of Fluoro-Jade C positive denegerating pyramidal cells and DAPI-positive viable pyramidal cells. Photo is courtesy of Dr. Larry Schmued.

Fluoro-Jade C (FJC) staining (green) of degenerating neurons in the CA3 region of the hippocampus in old diabetic rats. Blue: DAPI nuclear stain. Picture courtesy of Dr. Wang and colleagues. Results of this study were published in Wang S. et al. (2020), Geroscience, PMID: 32696219.

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Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

Flow, ICC, IHC

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Data Sheet

SKU: TR-100-FJ

Volume/Size: 40 mL

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$517.00

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Product Details

Product Description

The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.

Target

Degenerating neurons

Immunogen Species

Human

Specificity

Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.

Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

Flow, ICC, IHC

Application Details

The Fluoro-Jade C Ready-to-Dilute (RTD) Staining Kit provides an easy-to-use assortment of Fluoro-Jade C, DAPI, sodium hydroxide and potassium permanganate in liquid form. Following our detailed protocol, Fluoro-Jade C labelled degenerating neurons are visualized with blue light excitation while DAPI counter stained cell nuclei are visualized with ultra-violet illumination. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.

Method of Analysis

Fluorescence

Format

Purified

Physical State

Liquid, ready to dilute

Synonyms

FJC

Usage Statement

For research use only.

Country of Origin

United States

Storage

The unopened kit can be stored for up to 6 months at 2-8°C after the date of receipt. The kit and components should be stored protected from light. Diluted FJC dye solutions are not stable and should be used within 4 hours of making. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions. We recommend using aseptic techniques when handling the reagents to avoid bacterial growth and contamination.

The FJC Ready to dilute kit is shipped ambient and stable at room temperature during transport. Refrigerate upon arrival, do not freeze.

Shipping

Shipped on ice packs

Fluoro-Jade C Staining Kit Protocol

Materials provided:

Sodium Hydroxide, Solution A (Dilute 1:10 prior to use) - 20/40 mL (TR-100-FJT / TR-100-FJ)
Potassium Permanganate, Solution B (Dilute 1:10 prior to use) - 20/40 mL (TR-100-FJT / TR-100-FJ)
Fluoro-Jade C, Solution C (Dilute 1:10 prior to use) - 20/40 mL (TR-100-FJT / TR-100-FJ)
DAPI, Solution D (Add to diluted Fluoro-Jade C) - 20/40 mL (TR-100-FJT / TR-100-FJ)

Equipment and Reagents required:

Gelatin coated microscope slides
Staining dishes/Coplin jars
Cover slips
DPX mounting media
Slide warmer
Convection oven
Distilled water
Ethanol
Xylene

Number of slides processed:

The actual number of slides processed by this kit will depend largely upon the vessel that is used to incubate the slides. If using a standard Coplin Jar, its capacity is 50 mL and typically holds 5 slides per jar. If using such a device, then 80-100 slides stained per 50 ml of working solution (or, 5 ml of stock solution) could be processed in one day. Note the diluted dye is NOT stable and will not store overnight. It is best to use freshly diluted dye each time an experimental batch is started.

Final working concentrations of FJC: 0.0001%
Final working concentration of KMnO4: 0.06%

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Fluoro-Jade C (FJC) Staining Kit

SKU: TR-100-FJ

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