Black-Gold II Myelin Staining Kit

Name: Black-Gold II Myelin Staining Kit
Brand: Biosensis
SKU: TR-100-BG
Price: 697.0 USD
Availability: InStock

Bright field illumination (60X mag) of the dentate gyrus of a normal mouse reveals individual Black-Gold II stained myelinated fibres and Toluadine Blue O stained polymorph cells (centre) and granule cells (top). Photo is courtesy of Dr. Larry Schmued.

Bright field illumination (60X mag) of 8 month old Ad-Tg mouse hippocampus. The myelin pathology can be observed in and around amyloid plaques. The cell bodies of adjacent granule and polymorph cells appear blue, while individual myeliated fibres appear nearly black. Photo is courtesy of Dr. Larry Schmued.

Human brain Locus K (Del Fiacco et al., Brain Struct Funct. 2014, 2019: 2083-2101; Cells 2018, 7, 72): calcitonin gene-related peptide-like immunoreactivity (black) and myelin staining (red, Black-Gold II staining kit, Biosensis) in the medulla oblongata, showing the similar neurochemical and structural organization of the dorsal column nuclei Locus K and the spinal trigeminal substantia gelatinosa.

Bright field illumination (60X mag) of the dentate gyrus of a normal mouse reveals individual Black-Gold II stained myelinated fibres and Toluadine Blue O stained polymorph cells (centre) and granule cells (top). Photo is courtesy of Dr. Larry Schmued.

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Species Reactivity

All Mammals

Applications

IHC

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Data Sheet

SKU: TR-100-BG

Volume/Size: 10 mL

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$697.00

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Product Details

Product Description

Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, and co-localize cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black, while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination.

Black Gold II can demonstrate and characterize specific myelin changes associated with exposure to diverse neurotoxicants, including kainic acid, domoic acid, 3-nitropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers, including Nissl stains, retrogradely transported fluorescent tracers, and fluorescent markers for neuronal degeneration. The advantages of the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility. Biosensis' Ready-to-Dilute Staining (RTD) Black Gold II kit further advances this by making the use of Black Gold II easier to use with more consistency and cost-effectiveness. While the strict number of slide sections capable of being processed with a single TR-100-BG kit can vary because of the species, section thickness and myelin density present, this kit will typically efficiently process between 100-200 slides per 100 ml of Black Gold 1X solution and in many cases much more.

Target

Normal & pathogenic myelin

Immunogen Species

Species Independent

Specificity

Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system.

Species Reactivity

All Mammals

Applications

IHC

Application Details

Black Gold II is a high-resolution myelin stain with amyloid plaque counter stain. Its use is tailored to studies using formalin or paraformaldehyde fixed, non-paraffin embedded, non-solvent processed brain tissue. It can be used with both thick and thin sections. For thick sections, gelatin coated slides or slides specially designed to bind tissues sections should be use to avoid section loss. Free-floating sections can be used as well but sections are easier to handle and transfer when mounted on slides. A suggested method for thick sections is provided as a guide: Either frozen or vibratome sections are cut at a thickness of 20-50 μm and collected in 0.1 M neutral phosphate buffer. The sections are then typically mounted on 1% gel-coated slides and then air dried on a slide warmer (at 50°C) for at least an hour until thoroughly dried and adhered to the slide. The sections can be stained loose, although the sections are easier to handle when mounted on slides. The mounted sections were rehydrated in distilled water for 2 minutes before being processed in the staining solutions.

Method of Analysis

Colorimetric

Format

Purified

Physical State

Liquid, ready to dilute

Synonyms

BlackGold, Black and Gold

Usage Statement

For research use only.

Country of Origin

United States

Expiration

Unopened kit 6 months at 2-8°C protected from light

Storage

The kit can be transported at room temperature. Once received, the kit can be stored for up to 12 months at 2-8°C protected from light. Diluted solutions can be stored up to one month at 2-8°C protected from light.

Shipping

Shipped on ice packs

Black-Gold II Myelin Staining Kit Protocol

Black-Gold II (Dilute 1:10 prior to use) - 10 mL
Sodium Thiosulfate, fixative (Dilute 1:10 prior to use) - 10 mL
Toluidine Blue O (Dilute 1:10 prior to use) - 10 mL
Acetic Acid (Dilute 1:10 prior to use) - 10 mL

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Black-Gold II Myelin Staining Kit

SKU: TR-100-BG

Product Details

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