Anti-Polyubiqutin-B Antibody (Ubi-1)

Name: Anti-Polyubiqutin-B Antibody
Brand: Biosensis
SKU: M-1404-100
Price: 327.0 USD
Availability: InStock

Our Anti-Polyubiqutin-B mouse monoclonal primary antibody detects bovine, C. elegans, chicken, drosophila, and human Polyubiqutin-B, and is IgG. It is validated for use in IHC-Frozen, IHC-Paraffin-embedded, WB.

Mouse monoclonal antibody to Ubiquitin [Ubi-1] M-1404-100 staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.

Left: Analysis of ubiquitin expression (brown) in FFPE section of human cerebral cortex of an Alzheimer patient by Immunohistochemistry. Signal was generated with DAB. Counterstain: haemotoxylin (blue). A typical flame-shaped tangle is seen in a pyramidal neuron in the center and is surrounded by dystrophic neurites, also strongly ubiquitin-positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain. Right: Western blot analysis of HEK293 cell lysates for ubiquitin expression (green, 1:1,000). [1] protein standard, [2] cells maintained in normal medium, [3] cells treated with 10 uM of proteasome inhibitor lactacystin (Lc) for 16 hours. The smear detected above the 200 kDa standard represents accumulation of ubiquitinated proteins in proteasome inhibitor-Lc treated cells. The prominent band at 8 kDa corresponds to monoubiquitin. The same blot was probed with an antibody to HSP60 as loading control.

Mouse monoclonal antibody to Ubiquitin [Ubi-1] M-1404-100 staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.

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Species Reactivity

Bovine, Chicken, Drosophila, Human

Applications

IHC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1404-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Polyubiqutin-B

Scientific Background

Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).

Format

IgG

Clonality

Monoclonal

Clone

Ubi-1

Isotype

IgG1

Applications

IHC, WB

Host Species

Mouse

Molecular Weight

8.5 kDa

Immunogen

Raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin.

Immunogen Species

Human

Species Reactivity

Bovine, Chicken, Drosophila, Human

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:500

Application Details

This antibody can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. This antibody also works on paraffin embedded material. It also recognizes other ubiquinated inclusion bodies such as Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues.

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~8.5 kDa Ubiquitin. Hu, Bov, Chk, Drosophila, and C. elegans.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

RPS27A; UBA52; UBB; UBC; Polyubiquitin-B; Polyubiquitin-C;

UniProt ID

P0CG47

Shipping

25°C (ambient)

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Anti-Polyubiqutin-B Antibody (Ubi-1)

SKU: M-1404-100

Product Details

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