Anti-Parvalbumin Antibody (3C9)

Our Anti-Parvalbumin mouse monoclonal primary antibody detects human, mouse, and rat Parvalbumin, and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Left: Staining of parvalbumin in adult rat cerebellum floating section by Immunohistochemistry. Mouse antibody to parvalbumin (1:1,000, green) stains dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue: Nuclear DNA stain. Right: Western blot analysis of parvalbumin expression in rat skeletal muscle homogenate (Lane 1). The antibody (1:1,000) detects parvalbumin at ~12 kDa in homogenate (Lane 1) and His-tagged parvalbumin protein (Lane 2) at slightly higher molecular weight. The antibody does not cross-react with His-tagged proteins calretinin (Lane 3) and calbindin (Lane 4). Note that this antibody does not recognize parvalbumin in rat or mouse brain lysates on western blots.
Rat cerebellum section stained with mouse anti-parvalbumin (green, 1:1,000) and chicken anti-calbindin (C-1798-50, red, 1:2,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone, and so appear green.
Left: Staining of parvalbumin in adult rat cerebellum floating section by Immunohistochemistry. Mouse antibody to parvalbumin (1:1,000, green) stains dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue: Nuclear DNA stain. Right: Western blot analysis of parvalbumin expression in rat skeletal muscle homogenate (Lane 1). The antibody (1:1,000) detects parvalbumin at ~12 kDa in homogenate (Lane 1) and His-tagged parvalbumin protein (Lane 2) at slightly higher molecular weight. The antibody does not cross-react with His-tagged proteins calretinin (Lane 3) and calbindin (Lane 4). Note that this antibody does not recognize parvalbumin in rat or mouse brain lysates on western blots.
Left: Staining of parvalbumin in adult rat cerebellum floating section by Immunohistochemistry. Mouse antibody to parvalbumin (1:1,000, green) stains dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue: Nuclear DNA stain. Right: Western blot analysis of parvalbumin expression in rat skeletal muscle homogenate (Lane 1). The antibody (1:1,000) detects parvalbumin at ~12 kDa in homogenate (Lane 1) and His-tagged parvalbumin protein (Lane 2) at slightly higher molecular weight. The antibody does not cross-react with His-tagged proteins calretinin (Lane 3) and calbindin (Lane 4). Note that this antibody does not recognize parvalbumin in rat or mouse brain lysates on western blots.
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Human, Mouse, Rat
IHC, WB
Mouse

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Data Sheet

SKU: M-1813-100

Volume/Size: 100 µg
Price:
$327.00

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