Anti-Neuronal nuclei antigen (NeuN/FOX3) Antibody (1B7) (1B7)
Our Anti-Neuronal nuclei antigen [NeuN] / RNA binding protein fox-1 homolog 3 [FOX3] (NeuN/FOX3) mouse monoclonal primary antibody detects human, mouse, and rat Neuronal nuclei antigen [NeuN] / RNA binding protein fox-1 homolog 3 [FOX3] (NeuN/FOX3), and is IgG. It is validated for use in IF, ICC, IHC, WB.
![Image of a rat brain stem section by Immunofluorescence and Immunohistochemistry. The section was stained with M-377-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Clone 1B7, Mouse mAb, (green), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (red), and DAPI staining of nuclear DNA (blue). Product M-377-100 selectively stains nuclei and the proximal cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.](http://www.antibodiesinc.com/cdn/shop/files/m-377-100-ihc-1_120x120.jpg?v=1759273593)
![Analysis by western blot of NeuN/FOX3 expression in rodent tissue homogenates with M-377-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Clone 1B7, Mouse mAb, with doublet bands at approx 46kDa and 48kDa; additional uncharacterized bands are observed. Method: SDS-PAGE: denatured, reduced; Transfer: Tris-Glycine buffer; Membrane: PVDF (0.45 µm); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8°C (1/2000); Secondary antibody: anti-mouse-HRP (1/10000) 2 hours at RT; Detection: Chemiluminiscence.](http://www.antibodiesinc.com/cdn/shop/files/m-377-100-wb_120x120.jpg?v=1759273593)
![Image of formalin-fixed paraffin-embedded rat hippocampus section by Immunohistochemistry. The sections was stained with M-377-100, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Clone 1B7, Mouse mAb, (brown, 1:2,000) and counter stained with Hematoxylin (blue).](http://www.antibodiesinc.com/cdn/shop/files/m-377-100-ihc-2_120x120.jpg?v=1759273593)
![Image of a rat brain stem section by Immunofluorescence and Immunohistochemistry. The section was stained with M-377-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Clone 1B7, Mouse mAb, (green), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (red), and DAPI staining of nuclear DNA (blue). Product M-377-100 selectively stains nuclei and the proximal cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.](http://www.antibodiesinc.com/cdn/shop/files/m-377-100-ihc-1_1600x.jpg?v=1759273593)
Image of a rat brain stem section by Immunofluorescence and Immunohistochemistry. The section was stained with M-377-50, Neuronal nuclei antigen [NeuN] /RNA binding protein fox-1 homolog 3 [FOX3], (NeuN/FOX3), Clone 1B7, Mouse mAb, (green), and co-stained with product C-1382-50, Microtubule associated protein (MAP2), Chicken pAb, (red), and DAPI staining of nuclear DNA (blue). Product M-377-100 selectively stains nuclei and the proximal cytoplasm of neuronal cells, while product C-1382-50 labels dendrites and overlaps with Fox3/NeuN staining in the perikarya of neurons. Method: following transcardial perfusion with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained with the two antibodies.
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