Anti-Neurofilament light polypeptide (NF-L) Antibody

Our Anti-Neurofilament light polypeptide (NF-L) chicken polyclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgY preparation. It is validated for use in IF, ICC, IHC, WB.

Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb (red), and co-stained with product R-1388-50, Neurofilament heavy polypeptide phosphorylated (pNF-H), Rabbit pAb, (green). The Neurofilament Light (NF-L) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast, the phosphorylated Neurofilament Heavy (NF-H) has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.
Analysis by western blot of NF-L expression in different tissue lysates probed with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb, (green, 1:20,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: cow spinal cord. Note NF-L proteins are known to have slightly different apparent SDS-PAGE molecular weights across species.
Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb, (green, 1:2,000), and co-stained with product M-377-100, NeuN/Fox3, Clone 1B7, Mouse mAb, (red, 1:5000). IHC Method: Following transcardial perfusion of rat with 4% PFA, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained. The NF-L antibody stains perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while product M-377-100 stains the nuclei and proximal cytoplasm of neurons.
Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb (red), and co-stained with product R-1388-50, Neurofilament heavy polypeptide phosphorylated (pNF-H), Rabbit pAb, (green). The Neurofilament Light (NF-L) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast, the phosphorylated Neurofilament Heavy (NF-H) has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.
Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb (red), and co-stained with product R-1388-50, Neurofilament heavy polypeptide phosphorylated (pNF-H), Rabbit pAb, (green). The Neurofilament Light (NF-L) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast, the phosphorylated Neurofilament Heavy (NF-H) has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.
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Bovine, Horse, Human, Mouse, Pig, Rat
ICC, IF, IHC, WB
Chicken

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Data Sheet

SKU: C-1390-50

Volume/Size: 50 µL
Price:
$327.00

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