Anti-High-Mobility Group Protein 1 (HMGB1) Antibody (1F3)

Our Anti-High-mobility group protein 1 (HMGP-1) mouse monoclonal primary antibody detects bovine, human, mouse, other mammals (predicted), pig, and rat High-mobility group protein 1 (HMGP-1), and is IgG. It is validated for use in FC, ICC, WB.

A: HeLa cells stained with M-1702-100 (red), chicken polyclonal antibody to Vimentin (C-1409-50, green) and DNA (blue). The M-1702-100 antibody reveals strong nuclear staining which overlaps with the DNA stain. B: Blot of crude HeLa cell extract stained with M-1702-100. HMGB1 runs at an apparent molecular weight of 25 kDa. C: Analysis of HMGB1 expression in human euroblastoma SH-SY5Y cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to HMGB1 (cat # M-1702-100, 2μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Left: HMGB1 detection in HeLa cells by Immunocytochemistry. Cells were stained with mouse antibody to HMGB1 (red, 1:2,000 ), and co-stained with rabbit antibody to GAPDH (R-1701-100, green, 1:2,000). The HMGB1 antibody stains the chromatin binding protein HMGB1, which is localized in the nuclei. In contrast, the GAPDH antibody produces strong cytoplasmic staining. Right: Western blot analysis of HMGB1 expression (green) in cell lysates, using mouse antibody to HMGB1 at 1:2,000 dilution. [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y. The 25 kDa band corresponds to HMGB1 protein.
A: HeLa cells stained with M-1702-100 (red), chicken polyclonal antibody to Vimentin (C-1409-50, green) and DNA (blue). The M-1702-100 antibody reveals strong nuclear staining which overlaps with the DNA stain. B: Blot of crude HeLa cell extract stained with M-1702-100. HMGB1 runs at an apparent molecular weight of 25 kDa. C: Analysis of HMGB1 expression in human euroblastoma SH-SY5Y cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to HMGB1 (cat # M-1702-100, 2μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
A: HeLa cells stained with M-1702-100 (red), chicken polyclonal antibody to Vimentin (C-1409-50, green) and DNA (blue). The M-1702-100 antibody reveals strong nuclear staining which overlaps with the DNA stain. B: Blot of crude HeLa cell extract stained with M-1702-100. HMGB1 runs at an apparent molecular weight of 25 kDa. C: Analysis of HMGB1 expression in human euroblastoma SH-SY5Y cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to HMGB1 (cat # M-1702-100, 2μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
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Bovine, Human, Mouse, Pig, Rat
Flow, ICC, WB
Mouse

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Data Sheet

SKU: M-1702-100

Volume/Size: 100 µg
Price:
$327.00

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