Anti-Glial fibrillary acidic protein (GFAP) Antibody

Name: Anti-Glial fibrillary acidic protein (GFAP) Antibody
Brand: Biosensis
SKU: C-1373-50
Price: 327.0 USD
Availability: InStock

Our Anti-Glial fibrillary acidic protein (GFAP) chicken polyclonal primary antibody detects cat, human, mouse, other mammals (predicted), and rat Glial fibrillary acidic protein (GFAP), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

Mixed cultures of neurons and glia stained with Chicken polyclonal antibody to Glial Fibrillary Acidic Protein C-1373-50 (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

Left: Analysis of GFAP expression (green) in mouse hippocampus section by Immunohistochemistry. Section was stained with chicken anti-GFAP antibody (1:5,000) and co-stained with rabbit antibody to FOX3/NeuN (R-3770-100, 1:5,000, red). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% PFA, mouse brain was post fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The GFAP antibody stains a network of astroglial cells, while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons. Right: Western blot analysis of GFAP expression in whole rat (2) and mouse (3) brain lysates using chicken anti-GFAP antibody (1:5,000). The strong band at about 50 kDa corresponds to GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.

Mixed cultures of neurons and glia stained with Chicken polyclonal antibody to Glial Fibrillary Acidic Protein C-1373-50 (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

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Species Reactivity

Feline, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1373-50

Volume/Size: 50 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Glial fibrillary acidic protein (GFAP)

Scientific Background

Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.

Format

IgY preparation

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

50 kDa

Immunogen

Recombinant GFAP (expressed in E.coli) and native bovine GFAP

Immunogen Species

Human

Species Reactivity

Feline, Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:5000
ICC: 1:1000-1:5000

Application Details

ICC: Using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3 hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded): capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. Human, rat, mouse, feine. Predicted to react with other mammals.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Astrocyte; Glial fibrillary acidic protein; GFAP;

UniProt ID

Q28115

Shipping

25°C (ambient)

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Anti-Glial fibrillary acidic protein (GFAP) Antibody

SKU: C-1373-50

Product Details

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