Anti-Alpha-Synuclein Antibody (3H9)

Name: Anti-Alpha-Synuclein Antibody
Brand: Biosensis
SKU: M-1402-100
Price: 337.0 USD
Availability: InStock

Our Anti-Alpha-synuclein mouse monoclonal primary antibody detects bovine, chicken, horse, human, mouse, other mammals (predicted), pig, and rat Alpha-synuclein, and is IgG. It is validated for use in ICC, WB.

Mixed neuron-glial cultures stained with Mouse monoclonal antibody to alpha Synuclein [3H9] M-1402-100 (red) and Chicken polylclonal antibody to MAP2 C-1382-50 (green). The alpha Synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image.

Western Blot image with Mouse monoclonal antibody to alpha Synuclein [3H9] M-1402-100 on recombinant alpha-synuclein (left lane) and crude extract of mouse brain (right lane). Image courtesy Rogan Tinsley, Howard Florey Institute, University of Melbourne).

Mixed neuron-glial cultures stained with Mouse monoclonal antibody to alpha Synuclein [3H9] M-1402-100 (red) and Chicken polylclonal antibody to MAP2 C-1382-50 (green). The alpha Synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image.

Click on image to zoom

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1402-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Alpha-synuclein

Scientific Background

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. FUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.SUBUNIT: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones. SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane-bound in dopaminergic neurons. Also found in the nucleus. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist.

Format

IgG

Clonality

Monoclonal

Clone

3H9

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Molecular Weight

14-15 kDa

Immunogen

Recombinant full length human alpha synuclein expressed and purified from E. coli

Immunogen Species

Human

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, divide into single use aliquots and store at -20-80°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:1000-1:5000
IHC: 1:500-1:3000
ICC: 1:500-1:3000

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~14-15 kDa alpha synuclein protein. The epitope for 3H9 is in the region 61-95 which correspond to the Non-Amyloid beta Component of Alzheimer's disease amyloid (NAC) region. 3H9 will also bind human alpha-synuclein containing the A30P and A53T mutations. Human, horse, cow, pig, chicken, rat, mouse. Predicted to react with other mammalian tissue because of highly conserved nature of the protein.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Alpha synuclein; Non-A beta component of AD amyloid; Alpha-synuclein; Non-A4 component of amyloid precursor; Alpha-synuclein; NACP; isoform NACP140; alphaSYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid; Non A4 component of amyloid precursor; Non-A beta component of AD amyloid; Non-A-beta component of alzheimers disease amyloid; precursor of; Non-A4 component of amyloid precursor; Non-A4 component of amyloid; OTTHUMP00000218549; OTTHUMP00000218551; OTTHUMP00000218552; OTTHUMP00000218553; OTTHUMP00000218554; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease (autosomal dominant Lewy body 4; Parkinson disease familial 1; SNCA; Snca synuclein alpha (non A4 component of amyloid precursor; SYN; Synuclein alpha; Synuclein alpha 140; Synuclein alpha (non A4 component of amyloid precursor; SYUA_HUMAN

UniProt ID

P37840
UniProt Summary: Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

NCBI Gene ID

6622

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Anti-Alpha-Synuclein Antibody (3H9)

SKU: M-1402-100

Product Details

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