Our mouse SCNN1G/Gamma-Subunit Epithelial Sodium Channel monoclonal antibody from Antibodies Incorporated is produced in-house from clone 3c7. It detects human SCNN1G/Gamma-Subunit Epithelial Sodium Channel and is Protein A or Protein G purified. Works in ELISA, IHC, WB.
Western blot detection of SCNN1G/Gamma-Subunit Epithelial Sodium Channel using Anti-SCNN1G/Gamma-Subunit Epithelial Sodium Channel Antibody (Cat. CAM 003-07-010). Gel was loaded with HEK293 cells transfected with and without γENaC. Lane 1 loaded with 5µg, Lane 2 loaded with 10µg, Lane 3 loaded with 20 µg. Proteins were transferred to the membrane, blocked, and then washed with a buffer. Primary antibody was incubated with sample and then detected via ECL using a secondary antibody.
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
3c7
IgG1/k
ELISA, IHC, WB
Mouse
SCNN1G
74 kDa
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
CAM 003-07 was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).
Unconjugated
CAM 003-07 is specific for the inhibitory tract of human yENaC subunit. The epitope differs from that of CAM 005-02
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
