Anti-Fibronectin Antibody (A32)

Name: Anti-Fibronectin Antibody
Brand: Antibodies Incorporated
SKU: CSI 005-32-010
Price: 325.0 USD
Availability: InStock

Our mouse Fibronectin monoclonal antibody from Antibodies Incorporated is produced in-house from clone A32. It detects bovine and human Fibronectin and is Protein A or Protein G purified. Works in ELISA, IHC, IP, WB.

Anti-Fibronectin Antibody

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Species Reactivity

Bovine, Human

Applications

ELISA, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: CSI 005-32-010

Volume: 100 uL

Variant

Price:

$325.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Fibronectin

Scientific Background

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. (1)

Format

Protein A or Protein G purified

Concentration

1 mg/mL ±15%

Clonality

Monoclonal

Clone

A32

Isotype

IgG1/k

Applications

ELISA, IHC, WB

Host Species

Mouse

Gene Name

FN1

Molecular Weight

440 kDa glycoprotein dimer, with monomers running at 260 on SDS-PAGE

Immunogen

Bovine corneal endothelial cells

Immunogen Species

Bovine

Species Reactivity

Bovine, Human

Storage

2-8°C without exposure to light

Physical State

Liquid

Production Notes

BSA free

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Dilution Ranges

WB: 1:100

Application Details

CSI 005-32 can be used in ELISA. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody CSI 005-35. (1, 2, 3, 4) In Western blotting dilution guideline of 1/100 has proved successful. (1) CSI 005-32 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

Conjugate

Unconjugated

Specificity

CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. Epitope is located in the 40kD Hep II heparin-binding domain.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

FN1, fibronectin 1

UniProt ID

P07589

NCBI Gene ID

280794

Shipping

Shipped on ice packs

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Anti-Fibronectin Antibody (A32)

SKU: CSI 005-32-010

Product Details

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