Anti-Aprotinin Antibody (21)

Name: Anti-Aprotinin Antibody
Brand: Antibodies Incorporated
SKU: ABS 037-21-010
Price: 450.0 USD
Availability: InStock

Our mouse Aprotinin monoclonal antibody from Antibodies Incorporated is produced in-house from clone 21. It detects bovine Aprotinin and is Protein A or Protein G purified. Works in ELISA, WB.

Anti-Aprotinin Antibody

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Species Reactivity

Bovine

Applications

ELISA, WB

Host Species

Mouse

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Data Sheet

SKU: ABS 037-21-010

Volume: 100 uL

Variant

Price:

$450.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Aprotinin

Scientific Background

Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury.

Format

Protein A or Protein G purified

Concentration

1 mg/mL ±15%

Clonality

Monoclonal

Clone

Isotype

IgG1/k

Applications

ELISA, WB

Host Species

Mouse

Gene Name

PTI

Molecular Weight

6.5 kDa

Immunogen

Aprotinin from bovine lung

Immunogen Species

Bovine

Species Reactivity

Bovine

Storage

2-8°C without exposure to light

Physical State

Liquid

Production Notes

BSA free

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Dilution Ranges

ELISA: 1:1000

Application Details

ABS 037-21 (as capture antibody coated at 2 μg/mL) forms an antibody pair with ABS 037-18 (as biotinylated detection antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 μg/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. PBS-based buffers are unsuitable for this assay and 0.2 M glycine sodium buffer, pH 8.8, is recommended for washing and dilution buffers.

Conjugate

Unconjugated

Specificity

ABS 037-21 binds bovine aprotinin immobilized on a solid phase or free in solution. The epitope of ABS 037-21 is different from the epitopes of ABS 037-18 and ABS 037-35.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

PTI, BPI, PTI, pancreatic trypsin inhibitor

UniProt ID

P00974

NCBI Gene ID

404172

Shipping

Shipped on ice packs

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Anti-Aprotinin Antibody (21)

SKU: ABS 037-21-010

Product Details

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