Anti-APC-AAT Complexes, Neoepitope Antibody (APC-7)
Our mouse APC-AAT Complexes, Neoepitope monoclonal antibody from Antibodies Incorporated is produced in-house from clone APC-7. It detects human APC-AAT Complexes, Neoepitope and is Protein A or Protein G purified. Works in ELISA.
Immunohistochemistry detection of APC-AAT complexes, neoepitope in human fallopian tube tissue using Anti-APC-AAT Complexes, Neoepitope Antibody (Cat. ABS 001-07-010). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. ABS 001-07-010) was incubated with the sample.
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SKU: ABS 001-07-010
Ships: 1-2 business days
Product Details
APC-AAT Complexes, Neoepitope
Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/ml because of the very fast turnover, the proenzyme concentration is approximately 3 µg/ml. The activated protein C (APC) is inhibited by members of the serine protease inhibitor (serpin) family, of which α1-antitrypsin (AAT) and protein C inhibitor (PCI) are the most important.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
APC-7
IgG1/k
ELISA
Mouse
PROC
62 kDa proenzyme, 55 kDa active enzyme
Recombinant human activated protein C
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
ABS 001-07 reacts strongly with APC-AAT complexes in ELISA. It can be used in sandwich ELISA in combination with a polyclonal anti-protein C antiserum. Note, that the conformational neoepitope expressed in the APC-AAT complex can also be expressed in APC coated directly onto a high-binding microtiter plate.
Unconjugated
ABS 001-07 is specific for a conformation-dependent neoepitope that is expressed in activated protein C upon complex-formation with α1-antitrypsin. No reaction is seen to non-complexed α1-antitrypsin and only very little cross reaction to protein C zymogen. Note that specificity is calcium dependent.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
PROC, APC, PC, PROC1, THPH3, THPH4, vitamin K-dependent protein C, Protein C-Nagoya, activated protein C, anticoagulant protein C, autoprothrombin IIA, blood coagulation factor XIV, coagulation factor XIV, prepro-protein C, type I protein C
5624
Shipped on ice packs

