Apoptosis and Autophagy Kits

62 products

Apoptosis is a process of programmed cell death that occurs in multicellular organisms. It is a highly regulated and controlled process that occurs normally during development and aging as a homeostatic mechanism to maintain cell populations.

Autophagy plays a critical role in maintaining homeostasis by preventing the accumulation of damaged organelles by disassembling unnecessary or dysfunctional cells and cellular components. Autophagy occurs at low levels in the cell under normal conditions and can be rapidly upregulated during times of starvation or stress.

ICT offers a full line of products to detect apoptosis, including FLICA, FLIVO, Annexin kits and more; our Autophagy Assay - Red Fluorescent enables researchers to detect and monitor the in vitro development of autophagy in living cells.

Showing 25 - 48 of 62 products

Figure 1. Signals leading to activation of the NLRP3 inflammasome.

Figure 2. THP-1 cells were treated with PMA (5 ng/mL) followed by LPS (100 ng/mL for 1 hour, lower row of images), or were untreated (upper row of images), and were then immediately stained with 660-YVAD-FMK for 1 hour at 37°C (concurrent with treatment). Cells were then stained with Hoechst 33342 and imaged. Intracellular caspase-1 activity was detected using Cy5 (Ex 620/60, Em 700/75) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Data courtesy of Mrs. Tracy Murphy (ICT 230:32).

ImmunoChemistry Technologies Pyroptosis/Caspase-1 Assay - Far Red Fluorescent

$392.50

ImmunoChemistry Technologies Tumor Necrosis Factor beta

$378.95

ImmunoChemistry Technologies Tumor Necrosis Factor alpha

$699

Figure 1. Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

Figure 2. Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

ImmunoChemistry Technologies Apo Active PE - Caspase 3 Detection Kit

$533.50

Figure 1. U937 cells were stimulated with staurosporine for 5 hours, and then assayed for caspase 3/7 activity following the kit protocol.

ImmunoChemistry Technologies Apo 3/7 HTS Assay Kit

From $327.05

Figure 1. Balb/c mice were either inoculated with HSV-1 virus, or given a sham treatment. Seven days later, the mice were injected IV with either DyLight® 747-VAD-FMK (kit 9114), NIR-DyLight® 747 Free Dye (kit 9115), or no reagent. Strong caspase activity was seen in the brain of the HSV-1 infected / NIR-DyLight® 747 Tracer-treated mouse. The liver is the route of clearance for DyLight® Tracers. All mice show fluorescence signal in the tail where reagent is likely to pool after IV injection.

ImmunoChemistry Technologies Near Infrared DyLight® 747 Free Dye Control Assay

$318.90

Figure 1. Apoptotic tissues in murine tumor models were imaged in vivo with 747-VAD-FMK (Cat. 9114). Negative control (placebo) mice (left) and experimentally treated mice (right) were injected intravenously with DyLight® 747-VAD-FMK tracer and imaged non-invasively at various time points with the CRi Maestro™ imaging system. Apoptotic tumor tissues fluoresce bright blue in the experimental group subjects, indicating that the experimental treatment had an apoptotic effect.

ImmunoChemistry Technologies Near-Infrared DyLight® 747 Tracer in vivo Active Caspase (VAD) Assay

$461.95

Figure 1. Brain abscesses were induced in mice following inoculation of live S. aureus. Mice received i.v. injections of DyLight® 690-VAD-FMK tracer or DyLight® 690 Dye Control (Cat. 9113) 17 hours post-infection; brain tissue was imaged 1 hour later ex vivo using an IVIS® Spectrum™ (Caliper Life Sciences). Strong caspase activity was seen in brain abscesses using DyLight® 690-VAD-FMK tracer (right); minimal signal was detected in the DyLight® 690 Free Dye Control (left).

ImmunoChemistry Technologies Near Infrared DyLight® 690 Free Dye Control Assay

$318.90

Experimental design of Aim2WT or Aim2−/− bone marrow (BM) transplantation to Ldlr−/−:Mx1cre:c-mybfl/fl mice receiving i.v. DNA (top) and flow cytometry histograms (bottom) of caspase-1 activity(Assay kit, Cat no. 9122) in bone marrow recipients 24 h after i.v. DNA. Image from publication, CC-BY-4.0, PMID: 39112714.

ImmunoChemistry Technologies Near-Infrared DyLight® 690 Tracer in vivo Active Caspase (VAD) Assay

$461.95

Figure 1. Excitation and emission spectra. Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 630 nm. The emission spectrum (orange) was generated using an excitation of 540 nm. The probe optimally excites at 550-580 nm and has a peak emission at 590-600 nm.

Figure 2. Mice were given either a 0Gy or 15Gy whole lung dose of gamma irradiation. SR in vivo probe (Cat. 982/983) was injected and allowed to circulate for 18 hours prior to sacrifice. Paraffin sections were prepared, nuclei stained with DAPI (blue), and lungs imaged with a fluorescence microscope. Caspase activity (red) was induced in irradiated mice compared to control animals. Data courtesy of E. Hernady (University of Rochester Medical Center, Rochester, NY).

ImmunoChemistry Technologies Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay

From $216.40

Figure 1. Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.

Figure 2. Staurosporine (Cat. 6212) was injected into the forebrain of a female house sparrow to induce apoptosis. Approximately 20 hours later, FAM in vivo probe (Cat. 980/981) was intravenously injected into the jugular and allowed to circulate 30 minutes. The bird was sacrificed and frozen brain sections prepared. Neurons with active caspases fluoresce green. This image shows one apoptotic neuron at 100X. Data courtesy of Mr. Chris Thompson at the University of Washington, Seattle.

ImmunoChemistry Technologies Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay

From $215.85

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained using Swine Annexin V-Fluorescein Assay (Cat. 9142). Annexin V-Fluorescein labeled only 13.1% of control cells (UR + LR = 11.0% + 3.1%, left), whereas staurosporine induced Annexin V-Fluorescein labeling in 96.7% of cells (UR + LR = 32.7% + 64.0%, right). Staurosporine also significantly increased the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:19.

ImmunoChemistry Technologies Swine Annexin V-Fluorescein Apoptosis Assay Kit

$479.35

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Equine Annexin V-Fluorescein Assay (Cat. 9141). Equine Annexin V-Fluorescene labeled only 17.4% of the control cells (UR + LR = 10.3% + 7.1%, left); staurosporine induced Equine Annexin V-Fluorescene labeling in 97.6% of cells (UR + LR = 27.8% + 69.8%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:68.

ImmunoChemistry Technologies Equine Annexin V-Fluorescein Apoptosis Assay Kit

$479.35

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Chicken Annexin V-Fluorescein Assay (Cat. 9140). Chicken Annexin V-Fluorescein labeled only 16.9% of control cells (UR + LR = 13.2% + 3.7%, left); staurosporine induced Chicken Annexin V-Fluorescein labeling in 97% of cells (UR + LR = 46.6% + 50.4%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Murphy, ICT, 227:19.

ImmunoChemistry Technologies Chicken Annexin V-Fluorescein Apoptosis Assay Kit

$479.35

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained with Canine Annexin V-Fluorescein Assay (Cat. 9139). Annexin V-Fluorescein labeled only 15.6% of the control cells (UR + LR = 9.8% + 5.8%, left); staurosporine induced Annexin V-Fluorescein labeling in 97.3% of cells (UR + LR = 11.5% + 85.8%, right). Staurosporine did not significantly increase the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 224:69-71.

ImmunoChemistry Technologies Canine Annexin V-Fluorescein Apoptosis Assay Kit

$479.35

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, and then stained with Bovine Annexin V-Fluorescein (Cat. 9138). Bovine Annexin V-Fluorescein labeled only 17.6% of control cells (UR + LR = 13.5% + 4.1%, left); staurosporine induced Bovine Annexin V-Fluorescein labeling in 98.8% of cells (UR + LR = 15.3% + 83.5%, right). Staurosporine did not increase the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Hanson, ICT, 224:69-71.

ImmunoChemistry Technologies Bovine Annexin V-Fluorescein Apoptosis Assay Kit

$479.35

ImmunoChemistry Technologies Staurosporine

$96.30

ImmunoChemistry Technologies Camptothecin

From $34.95

Figure 1. Jurkat cells were treated with staurosporine (Positive, Induced, right histogram). Negative control cells were spiked with a DMSO vehicle control (Negative, Non-induced, left histogram). Cells were stained with SR-VAD-OPH for 1 hour, washed twice, and read on a flow cytometer. Only 8.0% of control cells displayed caspase activity, whereas staurosporine induced caspase activity in 90.3% of the experimental cells. This is a ratio of 11:1. Data courtesy of Ms. Tracy Hanson, ICT 092713.

Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis, then labeled with SR-VAD-OPH for 60 minutes. Cells were washed twice and wet-mount slides were prepared. A grey DIC image (left) was taken, and red fluorescence (right) was detected using a band pass filter (excitation at 565 nm, emission at 590-600 nm). SR-VAD-OPH reveals complete caspase activation in the form of red fluorescence in all cells. Data courtesy of Ms. Tracy Hanson, ICT (ICT111809; SR_OPH_111_17_18).

ImmunoChemistry Technologies Red Fluorescent SR-VAD-OPH in vitro Poly Caspase Apoptosis Detection Reagent

$584.60

Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, and then labeled with FAM-DEVD-OPH (Cat. 6356). Wet-mount slides were prepared and green fluorescence (right) was detected using a band pass filter (excitation at 488 nm, emission at 520 nm). FAM-DEVD-OPH reveals complete caspase-3/7 activation in the form of green fluorescence in all cells. The corresponding DIC image is shown (left). Data courtesy of Ms. Tracy Hanson, ICT (ICT111709; FAM-DEVD-OPH_in_59_60).

ImmunoChemistry Technologies Green Fluorescent FAM-DEVD-OPH in vitro caspase-3/7 Apoptosis Detection Reagent

$584.60

Figure 1. Jurkat cells were treated with DMSO (left) or staurosporine (right), labeled with FAM-VAD-OPH II, washed, and stained with PI. Four cell populations are seen: unstained live cells do not fluoresce (lower left); early apoptotic cells fluoresce green with FAM- VAD-OPH II (lower right); late apoptotic cells (upper right) fluoresce green (active caspases) and red (permeabilized cell membrane); and necrotic cells fluoresce red (upper left). Data of Dr. M. Olin, University of Minnesota.

ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH II in vitro poly Caspase Apoptosis Detection Reagent

$584.60

Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, then stained with FAM- VAD-OPH I (Cat. 6354) or FAM-VAD-FMK FLICA® (Cat. 92), washed and then read on a flow cytometer. Results with the OPH inhibitor (grey solid) are consistent with those achieved using the FMK inhibitor (green outline). FAM-VAD-OPH inhibitors mirror FAM-VAD-FMK analogs for apoptosis detection, and they may be less toxic and more stable. Data courtesy of Dr. M. Olin, University of Minnesota (ICT-202).

ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH I in vitro poly Caspase Apoptosis Detection Reagent

$584.60

ImmunoChemistry Technologies Annexin V-FITC Apoptosis Assay Kit

$282.05

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed on a Molecular Devices Gemini XPS 96-well fluorescence plate reader. The excitation spectrum (grey) was generated using an emission of 760 nm. The emission spectrum (red) was generated using an excitation of 600 nm.

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis inducing agent (right), then stained with 660-VAD-FMK (Cat. 9120) for 1 hour. Cells were washed twice and read on an Accuri C6 flow cytometer. Treatment with the negative control induced caspase activity in only 8.2% of cells (left), whereas treatment with staurosporine induced caspase activity in 96.5% of cells (right). This is a ratio of 12:1. Data courtesy of Mrs. Tracy Murphy, ICT, 213:48.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Poly (active) Caspase (VAD) Assay Kit

$270.85

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Apoptosis and Autophagy Kits

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