Pronase Reagent (Ready to Use)
Pronase is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Pronase Reagent (Ready to Use)
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Ships: 1-2 business days
- Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer.
- Remove buffer, add 2-4 drops of Pronase reagent to cover tissue section.
- Incubate at room temp. for 5 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5 minutes are sufficient at room temp. In some cases it may require 15 minutes at room temp).
- Discard Pronase reagent, wash 3-5 times with buffer.
- The tissue sections are ready for further IHC protocol.