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Confocal Z stack images taken from the 9–12 kHz cochlear region in noninjected control Myo7afl/fl (A), Myo7afl/fl Myo15-cre+/– (B), and Myo7afl/fl Myo15-cre+/– mice injected with AAV9-Myo7a (C) at P49–P54. AAV-Myo7a was injected between P13 and P15. Cochleae were labeled with antibodies against BK (Cat. 75-408, 1:200, magenta) and the IHC markers MYO7A (cyan) or MYO6 (gray). BK was almost complete absence in Myo7afl/fl Myo15-cre+/– mice (B). Image from publication, CC-BY-4.0. PMID: 39641274Immunoblot against crude membranes from adult rat brain (RBM), wild-type (MBM-WT) and BKAlpha knockout (MBM- BKaKO) mouse brains probed with L6/23 (left), L6/60 (middle) or N52A/42 (right) TC supe. Reproduced from PLoS One (2010 Wersinger et al, PMID 21079807).
Adult rat optic nerve immunofluorescence staining L11A/41 (5 μg/ml)=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband.Adult rat brain membrane immunoblot.
Adult rat brain striatum and globus pallidus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and KChIP3 KO mouse brains probed with K66/38 (left) and K55/82 (right) TC supe. Mouse samples courtesy of Takeshi Sakurai and Joseph Buxbaum (Mount Sinai School of Medicine).
Immunofluorescence staining transiently Kv1.6-transfected COS cells.Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.6-transfected (COS+) and untransfected (COS-) COS cells.
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University
ANA Speckled Positive Control
Anti-ADAM11 Antibody (BSA and Azide free)
Ad CMV Luciferase Adenovirus
Anti-Neuropeptide Y Antibody FL490 Conjugate
Anti-Mortalin/GRP75 Antibody FL650 Conjugate
Anti-GFP (Green Fluorescent Protein) Antibody
Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N81/37 (left) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Immunoblot against crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N81/2 (left) or N52A/42 (right) TC supe.Immunoblot against adult rat brain membranes (RBM) and membranes from GABABR2 wild-type (WT) and knockout (KO) mice probed with N81/2 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Joshua Walker and Stephen Moss (Tufts).
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with TrpC4 and probed with N77/15 TC supe.Adult rat hippocampus immunohistochemistry.
Coronal sections of rat cerebellum, stained with human patient autoantibodies (I, red) and anti-VAChT (J, green, Cat no 75-020, 1:300). Colocalization was not observed when mixing the anti-VAChT with the autoantibodies (merge K, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228 Adult rat brain immunohistochemistry.
N56/21 immunofluorescence staining of cerebellar cortex from FGF14 wild-type (left) and knockout (right) mice. ml: molecular layer; pc: Purkinje cell layer; igl: internal granule cell layer; wm: white matter.Double immunofluorescence staining of cultured rat hippocampal neurons with N56/21 (red) and MAP2 (green). Images courtesy of David Ornitz (Washington University SOM).

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