Anti-Slo1/BKAlpha Potassium Channel Antibody (L6/23)

Name: Anti-Slo1/BKAlpha Potassium Channel Antibody
Brand: NeuroMab
SKU: 75-408
Price: 329.0 USD
Availability: InStock

5 Citations

Our Anti-Slo1/BKAlpha potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L6/23. It is KO validated, detects mouse and rat Slo1/BKAlpha potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Confocal Z stack images taken from the 9–12 kHz cochlear region in noninjected control Myo7afl/fl (A), Myo7afl/fl Myo15-cre+/– (B), and Myo7afl/fl Myo15-cre+/– mice injected with AAV9-Myo7a (C) at P49–P54. AAV-Myo7a was injected between P13 and P15. Cochleae were labeled with antibodies against BK (Cat. 75-408, 1:200, magenta) and the IHC markers MYO7A (cyan) or MYO6 (gray). BK was almost complete absence in Myo7afl/fl Myo15-cre+/– mice (B). Image from publication, CC-BY-4.0. PMID: 39641274

Immunoblot against crude membranes from adult rat brain (RBM), wild-type (MBM-WT) and BKAlpha knockout (MBM- BKaKO) mouse brains probed with L6/23 (left), L6/60 (middle) or N52A/42 (right) TC supe. Reproduced from PLoS One (2010 Wersinger et al, PMID 21079807).

Maximum intensity projections of confocal z stacks taken from the apical cochlear region of Eps8−/− (top) and Eps8−/− rescued (bottom) mice using antibodies against BK channels carrying IK,f (red) and the hair-cell marker Myo7a (white). Image from publication CC-BY-4.0. PMID: 36034774

Confocal Z stack images taken from the 9–12 kHz cochlear region in noninjected control Myo7afl/fl (A), Myo7afl/fl Myo15-cre+/– (B), and Myo7afl/fl Myo15-cre+/– mice injected with AAV9-Myo7a (C) at P49–P54. AAV-Myo7a was injected between P13 and P15. Cochleae were labeled with antibodies against BK (Cat. 75-408, 1:200, magenta) and the IHC markers MYO7A (cyan) or MYO6 (gray). BK was almost complete absence in Myo7afl/fl Myo15-cre+/– mice (B). Image from publication, CC-BY-4.0. PMID: 39641274

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-408

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Slo1/BKAlpha potassium channel

Scientific Background

Calcium-activated potassium channel subunit alpha-1, Potassium Calcium-Activated Channel Subfamily M Alpha 1 or Slo1/BKalpha is encoded by the gene KCNMA1. BKalpha binds to one of 4 different beta subunits to form a channel in the calcium activated large conductance (MaxiK channel) family. This potassium channel is activated by membrane depolarization or an increase in cytosolic ca2+ and mediated the export of K+. BKalpha is involved with various cell processes including contraction of smooth muscle, regulation of transmitter release, innate immunity and regulation of membrane potential. Diseases associated with KCNMA1 include forms of Epilepsy and Developmental Delay Cerebellar Atrophy and Seizures.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L6/23

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Kcnma1 Kcnma

Molecular Weight

110-130 kDa

Immunogen

Fusion protein amino acids 690-1196 (cytoplasmic C-terminus) of mouse Slo1 (accession number Q08460) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2491105

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

IHC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Calcium-activated potassium channel subunit alpha-1 (BK channel) (BKCA alpha) (Calcium-activated potassium channel, subfamily M subunit alpha-1) (K(VCA)alpha) (KCa1.1) (Maxi K channel) (MaxiK) (Slo-alpha) (Slo1) (mSlo1) (Slowpoke homolog) (Slo homolog) (mSlo)

UniProt ID

Q08460-2

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blot: Mouse | Rat

Immunohistochemistry: Mouse
PMID	Dilution	Publication
39641274	1:500	O'Connor, AP, et al. 2024. In vivo AAV9-Myo7a gene rescue restores hearing and cholinergic efferent innervation in inner hair cells. JCI Insight, .
36034774	1:200	Jeng, J.Y., et al. 2022. AAV-Mediated Rescue of Eps8 Expression In Vivo Restores Hair-Cell Function in a Mouse Model of Recessive Deafness. Molecular Therapy Methods & Clinical Development, 355-370.
33151556	1:200	Carlton, A., et al. 2020. Loss of Baiap2l2 destabilizes the transducing stereocilia of cochlear hair cells and leads to deafness. The Journal of Physiology, 1173-1198.
20826678	1:500	Zorrilla de San Martín, J., et al. 2010. Ca(2+) and Ca(2+)-activated K(+) channels that support and modulate transmitter release at the olivocochlear efferent-inner hair cell synapse.. Journal of Neuroscience, 12157-12167.

Immunohistochemistry: Rat
PMID	Dilution	Publication
21079807	1:500	Wersinger, E., et al. 2010. BK channels mediate cholinergic inhibition of high frequency cochlear hair cells.. PLoS One, e13836.

Western Blot: Mouse
PMID	Dilution	Publication
21079807	not listed	Wersinger, E., et al. 2010. BK channels mediate cholinergic inhibition of high frequency cochlear hair cells.. PLoS One, e13836.

Western Blot: Rat
PMID	Dilution	Publication
21079807	not listed	Wersinger, E., et al. 2010. BK channels mediate cholinergic inhibition of high frequency cochlear hair cells.. PLoS One, e13836.

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Anti-Slo1/BKAlpha Potassium Channel Antibody (L6/23)

SKU: 75-408

Product Details

Product Specific References for Applications and Species

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