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Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Pex7 or untagged Kv2.1 plasmid and probed with N232/9 (left) and 19E10 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/7 (left) or N86/8 (right) TC supe.
KC rabbit anti- Kv2.1 Brain samples courtesy of Albee Messing, UW- Madison.Immunofluorescence of GFAP WT and KO mouse hippocampus with N206B/9 Brain samples courtesy of Albee Messing, UW- Madison.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged INPP4b or untagged Kv2.1 plasmid and probed with N171/17 (left) and N86/8 (right) TC supe.Immunoblot of Inpp4b+/+ or Inpp4b−/− mouse embryonic fibroblasts treated with vehicle or apilimod 10 nM for 48 h and assessed for protein levels of Inpp4b, LC3, and Beta actin as loading control. Image from publication CC-BY-4.0. PMID: 35760104
Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment.
Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).Transfected HEK293 cell immunofluorescence staining N15/39=green, DAPI=blue.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Serial dilutions of KLH-conjugated and BSA-conjugated peptides dotted onto membrane and probed with N132A/12 TC supe (left) and a mouse loading control (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) or untagged Kv2.1 plasmid and probed with N129/15 (left) and K89/41 (right) TC supes.
Bacterial fusion protein immunoblot.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.2 or untagged Kv2.1 plasmids and probed with N124B/38 TC supe.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc- tagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.Adult rat hippocampus immunohistochemistry.
Bacterially expressed GST fusion protein immunoblot.
NeuroMab Anti-GST Antibody
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Adult rat hippocampus immunohistochemistry.Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with V5-tagged AMIGO-1, AMIGO-2, AMIGO-3 or untagged Kv2.1 plasmid and probed with L86/36 TC supe (left) and Mouse anti-V5 (right.
Immunoblot versus RBM and lysates of WT and AMIGO-1 KO mouse brains probed with L86/33 (left), K89/34 (middle) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Juha Kuja-Panula (University of Helsinki, Finland).Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot versus lysates of whole rat brain (WBH) and of COS-1 cells transfected with human and rat MMP9 (hMMP9 and rMMP9, respectively) and Kv2.1 plasmids and probed with L51/82 (left), K89/34 (middle) and loading control (right) TC supe.
Cultured hippocampal neurons (9 DIV) double- Stained for MAP2 (red), L5/1 (green).Adult rat neocortex immunohistochemistry.
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
Adult whole rat brain homogenate immunoblot.Transfected cell immunoblot: extracts of COS-7 cells transiently transfected with SynCAM1, SynCAM2 or SynCAM3 plasmids and probed with L45/30 TC supe. Image courtesy of Dr. Philip Washbourne, Institute of Neuroscience, University of Oregon.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with BKBeta4 or Kv2.1 plasmids and probed with L18A/3 pure IgG at 10 mg/mL (left panel) or K89/41 TC supe (right panel).
Array tomography immunofluorescence of a single 70 nm section (left) and volume reconstruction of 8 serial sections (right) of LRWhite-embedded adult mouse cerebellum with L118/66 (VGAT, green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). Purkinje cells (PC) have a low level of VGAT immunoreactivity, and numerous inhibitory synaptic varicosities in molecular (M) and granule (G) layers are colabeled with VGAT and GAD2. Images courtesy of Kristina Micheva (Stanford).Adult rat brain hippocampus immunohistochemistry.

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