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Adult rat brain membrane immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/63 (left) or N52A/42 (right) TC supe.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against extracts of COS cells transiently transfected with untagged GlyRAlpha3L, GlyRAlpha3K or Kv2.1 plasmid probed with N424/45 (left) or K89/34 (right) TC supe.
Immunoblot against extracts of COS cells transiently transfected with untagged ANO5/TMEM16E, GFP- tagged ANO6/TMEM16F or untagged Kv2.1 plasmid probed with N421A/85 (left) or N86/38 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with untagged TRAK1, GFP-tagged TRAK2 or untagged Kv2.1 plasmid probed with N389/9 (left) or N52A/42 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and Ankyrin-R knockout (KO) mouse brains probed with N388A/10 (left) and K58/35 (right) TC supe. Mouse brains courtesy of Matthew Rasband (Baylor College of Medicine.
Immunoblot against extracts of COS cells transiently transfected with untagged TRAK1, GFP-tagged TRAK2 or untagged Kv2.1 plasmid probed with N390/43 (left) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
adult rat Whole brain immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA-tagged Kir6.2 or untagged Kv2.1 plasmid probed with N363/71 (left) or N52A/42 (right) TC supe.
Immunofluorescence staining of a node of Ranvier in adult rat sciatic nerve with N343/26 (green) and bIV-spectrin rabbit polyclonal (red). Image courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged LRRK1, LRRK2 or untagged Kv2.1 plasmid and probed with N341/12 (left) or N241A/34 (right) TC supe.Immunoblot versus LRRK1-immunoprecipitated extracts from WT and LRRK1 KO mouse heart probed with N341/12 TC supe. Data courtesy of Paul Davies and Dario Alessi (University of Dundee, Scotland, UK.
Immunoblot versus extracts of heart tissue from WT and SUR2 KO mice probed with N323A/31 TC supe. Data courtesy of William Coetzee and Margaret Rice, New York University SOM.
Serial dilutions of BSA-conjugated modified and unmodified nucleotides dotted onto membrane and probed with N297/59 TC supe (left) and a rabbit polyclonal control (right).
Immunoblot versus crude membrane preparations from adult rat brain (RBM) and Brevican wild-type (WT) and knockout (KO) mice and probed with N294A/6 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Matthew Rasband, Baylor College of Medicine.
Immunohistochemistry with antigen retrieval via citrate pretreatment.Adult rat hippocampus immunohistochemistry under standard protocol.
Immunofluorescence staining of zebrafish sections at 5 days post- fertilization with N286/74 (green) and Synapsin 1/2 rabbit polyclonal (red). Left: optic tectum (TeO), inner plexiform layer (asterisk) and photoreceptor layer (arrow). Right: synapse-rich axonal projections in optic tectum. Image courtesy of Philip Washbourne (University of Oregon).Immunoblot versus homogenates from adult zebrafish muscle (Non- neuronal), brain (Neuronal) and purified brain postsynaptic densities (PSD) probed with N286/74 TC supe. Image courtesy of Philip Washbourne (University of Oregon).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right).
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N270/47 (left) and N86/8 (right).
Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Immunoblot against adult rat brain membrane (RBM) or low- speed pellet (RBLSP) fractions denatured at 95oC for 5 min or 37oC for 15 min and probed with N253/32 (left) and K58/35 (right) TC supe.
Adult rat brain membrane (RBM) immunoblot.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with DsRed-tagged GABAARAlpha4, Flag-tagged GABAARAlpha6 or untagged Kv2.1 plasmid probed with N229A/32 (left), Mouse anti-Flag (middle) or N398A/34 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Lhx6.1 and untagged Kv2.1 plasmids and probed with N228A/16 (left) and 19E10 (right) TC supe.

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