Anti-Nav1.6 Na+ Channel Antibody (K87A/10)

Name: Anti-Nav1.6 Na+ Channel Antibody
Brand: NeuroMab
SKU: 75-026
Price: 385.0 USD
Availability: InStock

65 Citations

Our Anti-Nav1.6 Na+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K87A/10. It is KO validated, detects human, mouse, and rat Nav1.6 Na+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat optic nerve immunofluorescence staining K87A/10=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband (Baylor College of Medicine).

(Closeup) Adult rat optic nerve immunofluorescence staining K87A/10=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband (Baylor College of Medicine).

Cerebellar granule cells, 16 days in culture, obtained from wild-type (WT) and Nav1.6-null “Null” mice. K87A/10=green. DAPI=blue. Scale bar = 20 μm. Images courtesy of Drs. L. M. Sharkey and M. H. Meisler, University of Michigan School of Medicine.

Adult rat optic nerve immunofluorescence staining K87A/10=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband (Baylor College of Medicine).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-026

Volume: 100 µL

Variant

Price:

$385.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Nav1.6 Na+ channel

Scientific Background

Nav1.6 Na+ channel (sodium channel, voltage-gated, type 8, alpha subunit/ SCN8A) is a member of voltage-gated sodium ion channel subunit family. It is encoded by gene Scn8a in human.The channel switches between open and close conformation in response to the voltage difference accross the membrane. Nav1.6 Na+ channel is located at the nodes of Ranvier. It heavily participates in the regulation of nerve conduction velocity. It has been shown that Nav1.6 Na+ channel loss in roddents induces paralysis due to the failure of the neuromuscular junction (Wagnon et al., 2016).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K87A/10

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP

Host Species

Mouse

Gene Name

Scn8a

Molecular Weight

<200 kDa

Immunogen

Synthetic peptide 459-476 (intracellular interdomain loop I-II) of rat Nav1.6 (accession number O88420)

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2184197

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Sodium channel protein type 8 subunit alpha (Peripheral nerve protein type 4) (PN4) (Sodium channel 6) (NaCh6) (Sodium channel protein type VIII subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.6)

UniProt ID

O88420

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Feline | Human | Mouse | Rat
Western Blot: Mouse
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
26985738	1:200	González-Burguera, I., et al. 2016. Highly efficient generation of glutamatergic/cholinergic NT2-derived postmitotic human neurons by short-term treatment with the nucleoside analogue cytosine β-D-arabinofuranoside. . Stem cell research, 541-551.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32375064	not listed	Weinstock, N.I., et al. 2020. Macrophages expressing GALC improve peripheral Krabbe disease by a mechanism independent of cross-correction..  Neuron, 65-81.
30796290	1:300	Reijntjes, D.O., et al. 2019. Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice. . Scientific reports, 1-18.
28091722	1:200	Hatch, R.J., et al. 2017. Hyperphosphorylated tau causes reduced hippocampal CA1 excitability by relocating the axon initial segment.. Acta neuropathologica, 717-730.
20888080	1:500	Shepherd, M.N., et al. 2012. Paranodal reorganization results in the depletion of transverse bands in the aged central nervous system.. Neurobiology of Aging, 203-e13.
20861468	1:150	Tong, M., et al. 2010. The intrinsic electrophysiological properties of neurons derived from mouse embryonic stem cells overexpressing neurogenin-1.. American Journal of Physiology-Cell Physiology, C1335-C1344.
20133873	1:100	Brackenbury, W.J., et al. 2010. Functional reciprocity between Na+ channel Nav1. 6 and ß1 subunits in the coordinated regulation of excitability and neurite outgrowth. . Proceedings of the National Academy of Sciences, 2283-2288.
19261867	1:100	Sharkey, L.M., et al. 2009. The ataxia3 mutation in the N-terminal cytoplasmic domain of sodium channel Na(v)1.6 disrupts intracellular trafficking.. Journal of Neuroscience, 2733-2741.
19013247	1:200	O'Malley, H.A., et al. 2009. Loss of Na+ channel ß2 subunits is neuroprotective in a mouse model of multiple sclerosis. . Molecular and Cellular Neuroscience, 143-155.

Immunocytochemistry: Rat
PMID	Dilution	Publication
26408173	1:200	Barbosa, C., et al. 2015. Navß4 regulates fast resurgent sodium currents and excitability in sensory neurons.. Molecular Pain, s12990-015.
22885108	1:100	Devaux, J.J., et al. 2012. Antibodies to gliomedin cause peripheral demyelinating neuropathy and the dismantling of the nodes of Ranvier.. The American Journal of Pathology, 1402-1413.

Immunohistochemistry: Feline
PMID	Dilution	Publication
21543602	1:100	Meehan, C.F., et al. 2011. Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release.. Journal of Neuroscience, 6732-6740.

Immunohistochemistry: Human
PMID	Dilution	Publication
20838243	not listed	Howell, O.W., et al. 2010. Activated microglia mediate axoglial disruption that contributes to axonal injury in multiple sclerosis.. Journal of Neuropathology & Experimental Neurology, 1017-1033.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
39093969	1:100	Zhao, R, et al. 2024. Axo-axonic synaptic input drives homeostatic plasticity by tuning the axon initial segment structurally and functionally. Science Advances, eadk4331.
37283058	1:250	Dustin, E, et al. 2023. Adult-onset Depletion of Sulfatide Leads to Axonal Degeneration with Relative Myelin Sparing. Glia, 2285-2303.
36849716	1:100	Hu, A., et al. 2023. Projection-Specific Heterogeneity of the Axon Initial Segment of Pyramidal Neurons in the Prelimbic Cortex. Neuroscience Bulletin, .
35163623	1:300	Marosi, M., et al. 2022. Inhibition of the Akt/PKB Kinase Increases Nav1.6-Mediated Currents and Neuronal Excitability in CA1 Hippocampal Pyramidal Neurons. International journal of molecular sciences, 1700.
34825395	1:100	Newville, J., et al. 2020. Persistent myelin abnormalities in a third trimester‐equivalent mouse model of fetal alcohol spectrum disorder. . Alcoholism: Clinical and Experimental Research, 77-86.
32142125	not listed	Kawamura, A., et al. 2020. Oligodendrocyte dysfunction due to Chd8 mutation gives rise to behavioral deficits in mice.. Human molecular genetics, 1274-1291.
31933626	1:300	Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model. Neural Plasticity, 4893103.
30125271	not listed	Nunes, D., et al. 2018. Axonal sodium channel NaV1. 2 drives granule cell dendritic GABA release and rapid odor discrimination. . PLoS biology, e2003816.
28595650	1:200	Benusa, S.D., et al. 2017. Acute neuroinflammation induces AIS structural plasticity in a NOX2-dependent manner. . Journal of neuroinflammation, 1-14.
28355569	1:5 (supe)	Morelli, K.H., et al. 2017. Severity of demyelinating and axonal neuropathy mouse models is modified by genes affecting structure and function of peripheral nodes. . Cell reports, 3178-3191.
28283581	1:500	Satake, T., et al. 2017. MTCL1 plays an essential role in maintaining Purkinje neuron axon initial segme. The EMBO journal, 1227-1242.
27629714	1:100	Kim, S., et al. 2016. Schwann cell O-GlcNAc glycosylation is required for myelin maintenance and axon integrity.. Journal of Neuroscience, 9633-9646.
27581456	not listed	Marin, M.A., et al. 2016. Reassembly of excitable domains after CNS axon regeneration.. Journal of Neuroscience, 9148-9160.
26909021	1:300	Alshammari, M.A., et al. 2016. Improved methods for fluorescence microscopy detection of macromolecules at the axon initial segment. . Frontiers in cellular neuroscience, 5.
26807988	1:200	Jones, J.M., et al. 2016. Single amino acid deletion in transmembrane segment D4S6 of sodium channel Scn8a (Nav1. 6) in a mouse mutant with a chronic movement disorder.. Neurobiology of disease, 36-45.
25653382	not listed	Baalman, K., et al. 2015. Axon initial segment-associated microglia.. Journal of Neuroscience, 2283-2292.
25362471	not listed	Chang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-81.
23891806	1:1000	Xiao, M., et al. 2013. FGF14 localization and organization of the axon initial segment. . Molecular and Cellular Neuroscience, 393-403.
23864679	1:200	Ruff, C.A., et al. 2013. Effects of adult neural precursor-derived myelination on axonal function in the perinatal congenitally dysmyelinated brain: optimizing time of intervention, developing accurate prediction models, and enhancing performance.. Journal of Neuroscience, 11899-11915.
23090952	1:250	Sun, W., et al. 2013. Aberrant sodium channel activity in the complex seizure disorder of Celf4 mutant mice.. The Journal of Physiology, 241-255.
22492029	not listed	Buttermore, E.D., et al. 2012. Pinceau organization in the cerebellum requires distinct functions of neurofascin in Purkinje and basket neurons during postnatal development.. Journal of Neuroscience, 4724-4742.
21958379	not listed	Duflocq, A., et al. 2011. Characterization of the axon initial segment (AIS) of motor neurons and identification of a para-AIS and a juxtapara-AIS, organized by protein 4.1B.. BMC Biology, 1-19.
21525278	1:100	Binder, E., et al. 2011. Peripheral nervous system progenitors can be reprogrammed to produce myelinating oligodendrocytes and repair brain lesions.. Journal of Neuroscience, 6379-6391.
20838243	not listed	Howell, O.W., et al. 2010. Activated microglia mediate axoglial disruption that contributes to axonal injury in multiple sclerosis.. Journal of Neuropathology & Experimental Neurology, 1017-1033.
20628201	not listed	Wimmer, V.C., et al. 2010. Axon initial segment dysfunction in a mouse model of genetic epilepsy with febrile seizures plus. . The Journal of clinical investigation, 2661-2671.
20375142	not listed	Wimmer, V.C., et al. 2010. Axon initial segment dysfunction in epilepsy. . The Journal of physiology, 1829-1840.
20173079	1:200	Osorio, N., et al. 2010. Persistent Nav1.6 current at axon initial segments tunes spike timing of cerebellar granule cells.. The Journal of Physiology, 651-670.
20133873	1:100	Brackenbury, W.J., et al. 2010. Functional reciprocity between Na+ channel Nav1. 6 and ß1 subunits in the coordinated regulation of excitability and neurite outgrowth. . Proceedings of the National Academy of Sciences, 2283-2288.
18661556	1:1000	Desai, M.K., et al. 2009. Triple‐transgenic Alzheimer's disease mice exhibit region‐specific abnormalities in brain myelination patterns prior to appearance of amyloid and tau pathology. . Glia, 4-65.
18930825	1:1000	Shakkottai, V.G., et al. 2008. FGF14 regulates the intrinsic excitability of cerebellar Purkinje neurons.. Neurobiology of Disease, 81-88.
18621130	not listed	Duflocq, A., et al. 2008. Nav1. 1 is predominantly expressed in nodes of Ranvier and axon initial segments. . Molecular and Cellular Neuroscience, 180-192.
18522848	1:200	Windrem, M.S., et al. 2008. Neonatal chimerization with human glial progenitor cells can both remyelinate and rescue the otherwise lethally hypomyelinated shiverer mouse.. Cell Stem Cell, 553-565.

Immunohistochemistry: Rat
PMID	Dilution	Publication
25948259	1:200	Hirono, M., et al. 2015. BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells.. Journal of Neuroscience, 7082-7094.
24477962	1:100	King, A.N., et al. 2014. A unique ion channel clustering domain on the axon initial segment of mammalian neurons. . Journal of Comparative Neurology, 2594-2608.
21734302	1:200	Lysakowski, A., et al. 2011. Molecular microdomains in a sensory terminal, the vestibular calyx ending.. Journal of Neuroscience, 10101-10114.
19118165	1:500	Lorincz, A., et al. 2008. Cell-type-dependent molecular composition of the axon initial segment. . Journal of Neuroscience, 14329-14340.
18769561	1:500-1:1000	Mojumder, D.K., et al. 2008. Subcellular compartmentalization of two calcium binding proteins, calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina. . Molecular Vision, 1600.

Western Blot: Mouse
PMID	Dilution	Publication
31933626	1:500	Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model. Neural Plasticity, 4893103.
30125271	not listed	Nunes, D., et al. 2018. Axonal sodium channel NaV1. 2 drives granule cell dendritic GABA release and rapid odor discrimination. . PLoS biology, e2003816.
29491011	1:200	Zhou, L., et al. 2018. Celecoxib ameliorates seizure susceptibility in autosomal dominant lateral temporal epilepsy. . Journal of Neuroscience, 3346-3357.
26406915	not listed	Brügger, V., et al. 2015. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.. PLoS Biology, e1002258.
25362471	not listed	Chang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-81.
19013247	1:200	O'Malley, H.A., et al. 2009. Loss of Na+ channel ß2 subunits is neuroprotective in a mouse model of multiple sclerosis. . Molecular and Cellular Neuroscience, 143-155.
16870743	1:100	Lopez-Santiago, L.F., et al. 2006. Sodium channel beta2 subunits regulate tetrodotoxin-sensitive sodium channels in small dorsal root ganglion neurons and modulate the response to pain.. Journal of Neuroscience, 7984-7994.

Additional Publications: Unspecified
PMID	Publication
30500400	Brasileiro, A.D., et al. 2019. Effects of diabetes mellitus on myenteric neuronal density and sodium channel expression in the rat ileum. . Brain Research, 1-9.
27100937	Clark, K.C., et al. 2016. Compromised axon initial segment integrity in EAE is preceded by microglial reactivity and contact. Glia, 64(7), pp.1190-1209.. Glia, 1190-1209.
25421039	Wimmer, V.C., et al. 2015. Sodium channel ß1 subunit localizes to axon initial segments of excitatory and inhibitory neurons and shows regional heterogeneity in mouse brain.. Journal of Comparative Neurology, 814-830.
23025758	Baalman, K.L., et al. 2013. Blast wave exposure impairs memory and decreases axon initial segment length.. Journal of Neurotrauma, 741-751.
19800949	Crawford, D.K., et al. 2009. Functional recovery of callosal axons following demyelination: a critical window.. Neuroscience, 1407-1421.
18079688	Mojumder, D.K., et al. 2007. Voltage-gated sodium channel alpha-subunits Na(v)1.1, Na(v)1.2, and Na(v)1.6 in the distal mammalian retina.. Molecular Vision, 2163-2182.
17884088	Lopez-Santiago, L.F., et al. 2007. Sodium channel Scn1b null mice exhibit prolonged QT and RR intervals. . Journal of molecular and cellular cardiology, 636-647.
16817858	Rush, A.M., et al. 2006. Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily.. European Journal of Neuroscience, 2551-2562.

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Anti-Nav1.6 Na+ Channel Antibody (K87A/10)

SKU: 75-026

Product Details

Product Specific References for Applications and Species

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