Anti-GluN2C/NR2C Antibody (N422/18)

Name: Anti-GluN2C/NR2C Antibody
Brand: NeuroMab
SKU: 75-411
Price: 329.0 USD
Availability: InStock

Our Anti-GluN2C/NR2C mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N422/18. It is KO validated, detects human, mouse, and rat GluN2C/NR2C, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunofluorescence staining of sections from adult GluN2C/NR2C wild-type (WT) and knockout (KO) mouse cerebellum with N422/18 (GluN2C/NR2C, red) and L106/83 (Gephyrin, green) TC supe. Tissue courtesy of Sharon Swanger and Stephen Traynelis (Emory).

$Immunoblot versus crude membrane fractions from GluN2C/NR2C knockout (KO) and wild-type (WT) mouse cerebella probed with N422/18 (top) and tubulin (bottom). Data courtesy of Bo-Shiun Chen (Augusta University) and reproduced with permission from Scientific Reports (2016 Chung et al, PMID 27845401).$

Immunoblot from NAc synaptoneurosomal preparation of WT and GluN2C KO mice, confirming loss of GluN2C subunit. Image from publication CC-BY-4.0. PMID: 35867797

Immunofluorescence staining of sections from adult GluN2C/NR2C wild-type (WT) and knockout (KO) mouse cerebellum with N422/18 (GluN2C/NR2C, red) and L106/83 (Gephyrin, green) TC supe. Tissue courtesy of Sharon Swanger and Stephen Traynelis (Emory).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-411

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluN2C/NR2C

Scientific Background

Glutamate receptor ionotropic, NMDA 2C, Nmethyl D-aspartate receptor subtype 2C, or GluN2C/NR2C is encoded by the gene GRIN2C. GluN2C is a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of channel forming ionotropic glutamate receptor which is permeable to cations including calcium, potassium and sodium. GluN2C is found in the central nervous system and the cerebellum. GluN2C is thought to have an important role in physiological processes such as learning, memory, and synaptic development. Diseases associated with this gene include Alzheimer disease, Parkinson’s disease and Schizophrenia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N422/18

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Grin2c

Molecular Weight

130 kDa

Immunogen

Fusion protein amino acids 836-1233 (cytoplasmic C-terminus) of rat GluN2C (accession number Q00961) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2531892

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Glutamate receptor ionotropic, NMDA 2C (GluN2C) (Glutamate [NMDA] receptor subunit epsilon-3) (N-methyl D-aspartate receptor subtype 2C) (NMDAR2C) (NR2C)

UniProt ID

Q00961

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
35867797	1:500	Shelkar, G.P., et al. 2022. Cocaine Preference and Neuroadaptations are Maintained by Astrocytic NMDA Receptors in the Nucleus Accumbens. Science Advances, eabo6574.
33421565	1:1000	Liu, J., et al. 2021. Facilitation of GluN2C-containing NMDA receptors in the external globus pallidus increases firing of fast spiking neurons and improves motor function in a hemiparkinsonian mouse model. Neurobiology Disorders, 105254.
31110197	1:1000	Shelkar, G.P., et al. 2019. Differential effect of NMDA receptor GluN2C and GluN2D subunit ablation on behavior and channel blocker-induced schizophrenia phenotypes. Scientific Reports, 7572.

Western Blot: Rat
PMID	Dilution	Publication
27845401	not listed	Chung, C., et al. 2016. Neuroprotection Mediated through GluN2C-Containing N-methyl-D-aspartate (NMDA) Receptors Following Ischemia. Scientific Reports, 37033.

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Anti-GluN2C/NR2C Antibody (N422/18)

SKU: 75-411

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-GluN2C/NR2C Antibody

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