Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/20)
Our Anti-GluN2B/NR2B glutamate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N59/20. It is KO validated, detects human, mouse, and rat GluN2B/NR2B glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N59/20 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-097-n59-20-wb-1_120x120.jpg?v=1703184433)
Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).
Click on image to zoom
Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
KO Validated
Ships: 1-2 business days
Product Specific References for Applications and Species
- Immunohistochemistry: Mouse
- Immunoprecipitation: Human
- Western Blot: Mouse | Rat
- Additional Publications: Unspecified
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36672216 | 1:1000 | Ludewig, S., et al. 2023. Antibody Properties Associate with Clinical Phenotype in LGI1 Encephalitis. Cells, 282. |
| 29692709 | 1:100 | Gajardo, I., et al. 2018. Lack of pannexin 1 alters synaptic GluN2 subunit composition and spatial reversal learning in mice. Frontiers in Molecular Neuroscience, 114. |
| 24336719 | not listed | Gunn, B.G., et al. 2013. Dysfunctional astrocytic and synaptic regulation of hypothalamic glutamatergic transmission in a mouse model of early-life adversity: relevance to neurosteroids and programming of the stress response.. Journal of Neuroscience, 19534-54. |
| 23719805 | 1 : 1000 | Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384. |
| Immunoprecipitation: Human | ||
| PMID | Dilution | Publication |
| 39450669 | 10ul | Escamilla, S, et al. 2024. Synaptic and extrasynaptic distribution of NMDA receptors in the cortex of Alzheimer's disease patients. Alzheimer's & Dementia, . |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 38798824 | 1:1000 | Koster, KP, et al. 2024. Akap5 links synaptic dysfunction to neuroinflammatory signaling in a mouse model of infantile neuronal ceroid lipofuscinosis. Frontiers in Synaptic Neuroscience, 1384625. |
| 36681359 | 1:500 | Woodburn, S.C., et al. 2023. Depletion of Microglial BDNF Increases Susceptibility to the Behavioral and Synaptic Effects of Chronic Unpredictable Stress. Brain, Behavior, and Immunity, 127-138. |
| 36417513 | not listed | Sánchez-Castillo, C., et al. 2022. Functional Specialization Of Different PI3K Isoforms For The Control Of Neuronal Architecture, Synaptic Plasticity, And Cognition. Science Advances, eabq8109. |
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| 35953295 | 1:1000 | Angelini, C., et al. 2022. p140Cap Regulates the Composition and Localization of the NMDAR Complex in Synaptic Lipid Rafts. The Journal of Neuroscience, 7183-7200. |
| 34158618 | 1:500 | Seigneur, E., et al. 2021. Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors. Molecular Psychiatry, pp.1-13.. Molecular Psychiatry, 1-13. |
| 33436529 | not listed | Wang, Y.Q., et al. 2021. AMPA and NMDA receptor trafficking at cocaine-generated synapses. Journal of Neuroscience, 1996-2011. |
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| 32202499 | 1:1000 | Zhang, R.S., et al. 2020. Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum. eLife, e54443. |
| 31985401 | 1:1000 | Sclip, A., et al. 2020. LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses. eLife, e53406. |
| 29692709 | 1:500 | Gajardo, I., et al. 2018. Lack of pannexin 1 alters synaptic GluN2 subunit composition and spatial reversal learning in mice. Frontiers in Molecular Neuroscience, 114. |
| 29069439 | 1:100 | Shahin, S., et al. 2018. From the cover: 2.45-GHz microwave radiation impairs hippocampal learning and spatial memory: Involvement of local stress mechanism-induced suppression of …. Toxicological Sciences, 349-374. |
| 28840468 | 1:400 | Sanz-Blasco, S., et al. 2018. The Kinase Fyn As a Novel Intermediate in L-DOPA-Induced Dyskinesia in Parkinson's Disease. Molecular Neurobiology, 5125-5136. |
| 26946222 | 1:1000 | Yang, P., et al. 2016. Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist.. Experimental Neurology, 178-186. |
| 25653368 | not listed | Lo, S.C., et al. 2015. Caspase-3 deficiency results in disrupted synaptic homeostasis and impaired attention control.. Journal of Neuroscience, 2118-2132. |
| 24448406 | not listed | Li, Q., et al. 2014. The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation.. eLife, e01201. |
| 23719805 | 1 : 1000 | Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 39801227 | 1:400 | Vinci, E, et al. 2025. Regulation of Dendrite and Dendritic Spine Formation by TCF20. Journal of neurochemistry, e16297. |
| 35862168 | 1:500 | Zhou, J.J., et al. 2022. Calcineurin Controls Hypothalamic NMDA Receptor Activity and Sympathetic Outflow. Circulation Research, 345-360. |
| 33184769 | 1:250 | Barhorst, K.A., et al. 2020. Remote and Persistent Alterations in Glutamate Receptor Subunit Composition Induced by Spreading Depolarizations in Rat Brain. Cellular and Molecular Neurobiology, 1253-1260. |
| 31439005 | 1:125 | Dosemeci, A., et al. 2019. Postsynaptic densities fragment into subcomplexes upon sonication. Molecular Brain, 72. |
| 29496640 | not listed | Ji, Y., et al. 2018. Opposing roles of estradiol and testosterone on stress-induced visceral hypersensitivity in rats. The Journal of Pain, 764-776. |
| 29490268 | 1:1000 | Chen, J., et al. 2018. The α2δ-1-NMDA Receptor Complex Is Critically Involved in Neuropathic Pain Development and Gabapentin Therapeutic Actions. . Cell Reports, 2307–2321. |
| 27271196 | 1:1000 | Gross, G.G., et al. 2016. An E3-ligase-based method for ablating inhibitory synapses. Nature Methods, 673-678. |
| 25009255 | 1:200 | Kehoe, L.A., et al. 2014. GluN3A promotes dendritic spine pruning and destabilization during postnatal development.. Journal of Neuroscience, 9213-9221. |
| 24103031 | 1:500 | Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. FEBS Journal, 6114-27. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 26627310 | Berg, J.M., et al. 2015. JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.. Neuron, 1173-1191. | |
| 26557018 | Kim, S., et al. 2015. Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity.. The Korean Journal of Physiology and Pharmacology, 515-522. | |
| 26203142 | Zollinger, D.R., et al. 2015. The Polarity Protein Pals1 Regulates Radial Sorting of Axons.. Journal of Neuroscience, 10474-10484. | |
| 25755642 | McGuier, N.S., et al. 2015. Homer2 deletion alters dendritic spine morphology but not alcohol-associated adaptations in GluN2B-containing N-methyl-D-aspartate receptors in the nucleus accumbens.. Frontiers in Pharmacology, 28. | |
| 25707359 | Chen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes.. Biochimica et Biophysica Acta, 827-833. | |
| 25698751 | Chassefeyre, R., et al. 2015. Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B.. Journal of Neuroscience, 3155-3173. | |
| 25632145 | D'Orsi, B., et al. 2015. Bax regulates neuronal Ca2+ homeostasis.. Journal of Neuroscience, 1706-1722. | |
| 25513972 | Neuner, S.M., et al. 2015. TRPC3 channels critically regulate hippocampal excitability and contextual fear memory.. Behavioural Brain Research, 69-77. | |
| 25468278 | McGuier, N.S., et al. 2015. Withdrawal from chronic intermittent alcohol exposure increases dendritic spine density in the lateral orbitofrontal cortex of mice.. Alcohol, 21-27. | |
| 25319628 | Mellone, M., et al. 2015. Zinc transporter-1: a novel NMDA receptor-binding protein at the postsynaptic density.. Journal of Neurochemistry, 159-168. | |
| 25069643 | Bhattacharyya, M., et al. 2015. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens.. Neurochemical Research, 1825-1832. | |
| 24728870 | Neumann, J.R., et al. 2015. Y-P30 promotes axonal growth by stabilizing growth cones.. Brain Structure and Function, 1935-1950. | |
| 25505316 | Stroebel, D., et al. 2014. Controlling NMDA receptor subunit composition using ectopic retention signals.. Journal of Neuroscience, 16630-16636. | |
| 25352748 | de Andrade, G.B., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.. Molecular Vision, 1422-1433. | |
| 24599469 | Trantham-Davidson, H., et al. 2014. Chronic alcohol disrupts dopamine receptor activity and the cognitive function of the medial prefrontal cortex.. Journal of Neuroscience, 3706-3718. | |
| 24403152 | Portugal, G.S., et al. 2014. Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference.. Journal of Neuroscience, 527-538. | |
| 24297929 | Fiuza, M., et al. 2013. GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling.. Proceedings of the National Academy of Science of the United States of America, 20807-20812. | |
| 24260577 | Kunda, S., et al. 2013. Antagonist properties of Conus parius peptides on N-methyl-D-aspartate receptors and their effects on CREB signaling.. PLoS One, e81405. | |
| 24259588 | Louveau, A., et al. 2013. Impaired Spatial Memory in Mice Lacking CD3ζ Is Associated with Altered NMDA and AMPA Receptors Signaling Independent of T-Cell Deficiency. Journal of Neuroscience, 18672-18685. | |
| 24244696 | den Hartog, C.R., et al. 2013. Alterations in ethanol-induced behaviors and consumption in knock-in mice expressing ethanol-resistant NMDA receptors.. PLoS One, e80541. | |
| 24183704 | Yuan, T., et al. 2013. Expression of cocaine-evoked synaptic plasticity by GluN3A-containing NMDA receptors.. Neuron, 1025-1038. | |
| 24069373 | Breiderhoff, T., et al. 2013. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction.. PLoS One, e75006. | |
| 24068818 | Krüger, J.M., et al. 2013. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.. Journal of Neuroscience, 15504-15517. | |
| 24060584 | Viviani, B., et al. 2013. Early maternal deprivation immunologically primes hippocampal synapses by redistributing interleukin-1 receptor type I in a sex dependent manner.. Brain, Behavior, and Immunity, 135-143. | |
| 24056996 | Barcomb, K., et al. 2013. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B.. Molecular Pharmacology, 834-843. | |
| 24032518 | Gaier, E.D., et al. 2013. Peptidylglycine α-amidating monooxygenase heterozygosity alters brain copper handling with region specificity.. Journal of Neurochemistry, 605-619. | |
| 23946397 | Bonnet, S.A., et al. 2013. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409. | |
| 23874151 | Frühbeis, C., et al. 2013. Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.. PLoS Biology, e1001604. | |
| 23852340 | Marco, S., et al. 2013. Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington''s disease models.. Nature Medicine, 1030-1038. | |
| 23831965 | Brigman, J.L., et al. 2013. GluN2B in corticostriatal circuits governs choice learning and choice shifting.. Nature Neuroscience, 1101-1110. | |
| 23486974 | Murata, Y., et al. 2013. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.. Journal of Neuroscience, 5040-5052. | |
| 23447623 | Chowdhury, D., et al. 2013. Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.. Journal of Neuroscience, 4151-4164. | |
| 23295855 | Di Filippo, M., et al. 2013. Effects of central and peripheral inflammation on hippocampal synaptic plasticity.. Neurobiology of Disease, 229-236. | |
| 22784608 | Xiao, X., et al. 2013. Estrogen in the anterior cingulate cortex contributes to pain-related aversion.. Cerebral Cortex, 2190-2203. | |
| 23223310 | Bagetta, V., et al. 2012. Rebalance of striatal NMDA/AMPA receptor ratio underlies the reduced emergence of dyskinesia during D2-like dopamine agonist treatment in experimental Parkinson''s disease.. Journal of Neuroscience, 17921-17931. | |
| 23112146 | Harraz, M.M., et al. 2012. MicroRNA-223 is neuroprotective by targeting glutamate receptors.. Proceedings of the National Academy of Science of the United States of America, 18962-18967. | |
| 22989522 | Mazzone, C.M., et al. 2012. Analysis of kalirin-7 knockout mice reveals different effects in female mice.. Molecular Pharmacology, 1241-1249. | |
| 22869379 | Zhang, Y., et al. 2012. Estrogen facilitates spinal cord synaptic transmission via membrane-bound estrogen receptors: implications for pain hypersensitivity.. The Journal of Biological Chemistry, 33268-33281. | |
| 22703994 | Mulholland, P.J., et al. 2012. Chronic ethanol up-regulates the synaptic expression of the nuclear translational regulatory protein AIDA-1 in primary hippocampal neurons.. Alcohol, 569-576. | |
| 22666364 | Kroener, S., et al. 2012. Chronic Alcohol Exposure Alters Behavioral and Synaptic Plasticity of the Rodent Prefrontal Cortex. PLoS One, e37541. | |
| 22369234 | Wilke, S.A., et al. 2012. NeuroD2 regulates the development of hippocampal mossy fiber synapses.. Neural Development, 9. | |
| 22153375 | Wang, C.C., et al. 2011. A critical role for GluN2B-containing NMDA receptors in cortical development and function.. Neuron, 789-805. | |
| 21985178 | Peng, X., et al. 2011. Neuronal Nogo-A regulates glutamate receptor subunit expression in hippocampal neurons.. Journal of Neurochemistry, 1183-1193. | |
| 21880917 | Kiraly, D.D., et al. 2011. Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function.. Journal of Neuroscience, 12554-12565. | |
| 21708909 | Eipper-Mains, J.E., et al. 2011. microRNA-Seq reveals cocaine-regulated expression of striatal microRNAs.. RNA., 1529-1543. | |
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| 21135233 | Fourgeaud, L., et al. 2010. MHC class I modulates NMDA receptor function and AMPA receptor trafficking.. Proceedings of the National Academy of Science of the United States of America, 22278-22283. | |
| 20826662 | Hsin, H., et al. 2010. Proline-rich tyrosine kinase 2 regulates hippocampal long-term depression.. Journal of Neuroscience, 11983–11993. | |
| 20678574 | Antion, M.D., et al. 2010. Ephrin-B3 regulates glutamate receptor signaling at hippocampal synapses.. Molecular and Cellular Neuroscience, 378-388. | |
| 20220006 | Perez de Arce, K., et al. 2010. Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation.. Journal of Neuroscience, 3728-3738. | |
| 20159450 | Edbauer, D., et al. 2010. Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132.. Neuron, 373-384. | |
| 19605640 | Longordo, F., et al. 2009. NR2A at CA1 synapses is obligatory for the susceptibility of hippocampal plasticity to sleep loss.. Journal of Neuroscience, 9026-9041. | |
| 19135470 | Ghasemzadeh, M.B., et al. 2009. Locomotor sensitization to cocaine is associated with distinct pattern of glutamate receptor trafficking to the postsynaptic density in prefrontal cortex: early versus late withdrawal effects.. Pharmacology Biochemistry Behavior, 383-392. | |
| 19105975 | Ghasemzadeh, M.B., et al. 2009. Behavioral sensitization to cocaine is associated with increased glutamate receptor trafficking to the postsynaptic density after extended withdrawal period.. Neuroscience, 414-426. | |
| 19020030 | Ma, X.M., et al. 2008. Kalirin-7 is required for synaptic structure and function.. Journal of Neuroscience, 12368-12382. | |