Anti-Ankyrin-G (Blotting) Antibody (N106/20)

Name: Anti-Ankyrin-G (Blotting) Antibody
Brand: NeuroMab
SKU: 75-187
Price: 329.0 USD
Availability: InStock

27 Citations

Our Anti-Ankyrin-G (Blotting) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N106/20. It detects human, mouse, and rat Ankyrin-G (Blotting), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Lysates of rat cortical neurons treated with the indicated compounds were subjected to ABE to purify palmitoylated proteins. Levels of palmitoyl-AnkG-190 (right blot) and total AnkG (Cat. 75-187) expression in parent lysates (left blot bottom blot) were detected with specific antibodies. Exclusion of NH2OH was used as a control for assay specificity. Image from publication CC-BY-4.0. PMID:36969554

Representative field of view (FOV) for near-simultaneous recording of calcium activity (Fluo-4) and axonal mitochondrial transport (mitoTracker Red) of primary mouse hippocampal neurons in sparse cultures. Arrows mark axonal fiber. Inset: MAP2 (green), AnkG (magenta) and Tau immunolabeling to verify axonal identity and origin. Arrow indicates axon initial segment. Dotted line indicates axonal path. image from publication, CC-BY-4.0. PMID: 39698051.

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-187

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ankyrin-G (Blotting)

Scientific Background

Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS). Variants in Ankyrin G have been linked to bipolar disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N106/20

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

ANK3

Molecular Weight

>200 kDa

Immunogen

Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2057718

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.115

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Ankyrin-3 (ANK-3) (Ankyrin-G)

UniProt ID

E9PE32

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Mouse | Non-Human Primate | Rat
STORM: Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
39698051	1:250	Venneman, T, et al. 2024. Neuronal activity inhibits mitochondrial transport only in synaptically connected segments of the axon. Frontiers in Cellular Neuroscience, 1509283.
26024478	1:50	Yamankurt, G., et al. 2015. Exon organization and novel alternative splicing of Ank3 in mouse heart.. PLoS One, e0128177.
22632975	not listed	Galiano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-1139.

Immunocytochemistry: Rat
PMID	Dilution	Publication
31910261	not listed	Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
31474508	not listed	Fréal, A., et al. 2019. Feedback-driven assembly of the axon initial segment. Neuron, 305-321.
30723396	1:1000	Alpizar, S.A., et al. 2019. Loss of neurofascin-186 disrupts alignment of AnkyrinG relative to its binding partners in the axon initial segment. Frontiers in Cellular Neuroscience, 1.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32917708	1:200	Werginz, P., et al. 2020. Tailoring of the axon initial segment shapes the conversion of synaptic inputs into spiking output in OFF-α T retinal ganglion cells. Science Advances, eabb6642.
32736374	1:200	Werginz, P., et al. 2020. The relationship between morphological properties and thresholds to extracellular electric stimulation in α RGCs. Journal of Neural Engineering, 045015.
32726631	1:200	Holderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, 107968.
31910261	not listed	Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
29676260	1:500	Galliano, E., et al. 2018. Embryonic and postnatal neurogenesis produce functionally distinct subclasses of dopaminergic neuron. Elife, e32373.
22632975	not listed	Galiano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-1139.

Immunohistochemistry: Non-Human Primate
PMID	Dilution	Publication
24107939	1:3000	Puthussery, T., et al. 2013. NaV1. 1 channels in axon initial segments of bipolar cells augment input to magnocellular visual pathways in the primate retina. . Journal of Neuroscience, 16045-16059.

Immunohistochemistry: Rat
PMID	Dilution	Publication
31934859	1:500	Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na+ and KATP channels to the rat cardiac intercalated disc. Elife, e52373.
26195803	1:500	Wefelmeyer, W., et al. 2015. Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output.. PNAS: USA, 9757-9762.

STORM: Rat
PMID	Dilution	Publication
31934859	1:50	Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na+ and KATP channels to the rat cardiac intercalated disc. Elife, e52373.

Western Blot: Human
PMID	Dilution	Publication
27356871	not listed	Sohn, P.D., et al. 2016. Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment.. Molecular Neurodegeneration, 47.
24462599	not listed	Trinh-Trang-Tan, M.M., et al. 2014. AlphaII-spectrin participates in the surface expression of cell adhesion molecule L1 and neurite outgrowth.. Exp Cell Research, 365-380.

Western Blot: Mouse
PMID	Dilution	Publication
31910261	not listed	Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
30846310	1:200	Tai, Y., et al. 2019. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron, 358-372.
29956586	1:500	Dash, B., et al. 2018. Nonmuscle myosin II isoforms interact with sodium channel alpha subunits. . Molecular pain, 1744806918788638.
22632975	not listed	Galiano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-1139.

Western Blot: Rat
PMID	Dilution	Publication
36969554	not listed	Piguel, N.H., et al. 2023. Palmitoylation Controls the Stability of 190 kDa Ankyrin-G in Dendritic Spines and is Regulated by ZDHHC8 and Lithium. Frontiers in Molecular Neuroscience, 1144066.
31934859	1:2000	Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na+ and KATP channels to the rat cardiac intercalated disc. Elife, e52373.
23025758	not listed	Baalman, K.L., et al. 2013. Blast wave exposure impairs memory and decreases axon initial segment length.. Journal of Neurotrauma, 741-751.
21893642	1:200	Roussos, P., et al. 2011. Molecular and genetic evidence for abnormalities in the nodes of Ranvier in schizophrenia.. Archives Of General Psychiatry, 7-15.
21617128	not listed	Sato, P.Y., et al. 2011. Interactions between ankyrin-G, Plakophilin-2, and Connexin43 at the cardiac intercalated disc.. Circulation Research, 193-201.

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Anti-Ankyrin-G (Blotting) Antibody (N106/20)

SKU: 75-187

Product Details

Product Specific References for Applications and Species

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