Amylo-Glo® Amyloid Plaque Stain

Name: Amylo-Glo® Amyloid Plaque Stain
Brand: Biosensis
SKU: TR-300-AG
Price: 417.0 USD
Availability: InStock

Amylo-Glo® Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections.

Amylo-Glo® is a registered trademark of Histo-Chem.

This triple exposure allows for the simultaneous localization of Amylo-Glo® positive amyloid plaques (blue), GFAP positive hypertrophied astrocytes (green) and activated microglia (red) in the hippocampus of the AD/Tg mouse.Combined UV, blue and green light illumination.

Staining of amyloid plaques (blue) in J20 line heterozygote adult mouse brain. Image represents 3-D reconstruction from confocal z-stack. Red: astrocytes stained for GFAP. Green: Aggregates of cofilin rods. Method: brain was fixed by perfusion with 4% paraformaldehyde cut at 30 um thickness on freezing microtome, and stained as free floating sections. Picture courtesy of Dr. John Chilton, University of Exeter, www.axonology.com.

This triple exposure allows for the simultaneous localization of Amylo-Glo® positive amyloid plaques (blue), GFAP positive hypertrophied astrocytes (green) and activated microglia (red) in the hippocampus of the AD/Tg mouse.Combined UV, blue and green light illumination.

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Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

ICC, IHC

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Data Sheet Safety Data Sheet

SKU: TR-300-AG

Volume/Size: 5 mL

Variant

Price:

$417.00

Quantity:

Ships: 1-5 business days

Product Details

Product Description

Amylo-Glo® Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo® results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo® RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo® fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).

Target

Amyloid plaque

Immunogen Species

Species Independent

Specificity

Amyloid plaques both intraneuronal and vascular.

Species Reactivity

All Mammals, Human, Mouse, Rat

Applications

ICC, IHC

Application Details

Staining of amyloid plaques in human and animal tissues, see included protocol.

Method of Analysis

Fluorescence

Format

Purified

Physical State

Liquid, ready to dilute

Production Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.

Synonyms

AmyloGlo

Usage Statement

For research use only.

Country of Origin

United States

Expiration

6 months after date of receipt (unopened vial).

Storage

The stock solution can be stored for up to 6 months after date of receipt at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.

Shipping

Shipped on ice packs

Amylo-Glo Amyloid Plaque Stain Reagent Protocol

5 mL of 100X Amylo-Glo® RTD (A-G RTD) solution

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Amylo-Glo® Amyloid Plaque Stain

SKU: TR-300-AG

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