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Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine to induce apoptosis, then stained with TMRE, and photographed using a photomicroscope using a green excitation filter at 510-560 nm and a 570-620 nm emission filter (left). Healthy cells, containing mitochondria with polarized inner membranes fluoresce bright orange (A) compared with apoptotic cells with depolarized mitochondria(B). The corresponding DIC images are shown (right) (Dr. Brian Lee, ICT).

Figure 2. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRE - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$305.60

Figure 1. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

Figure 2. Jurkat cells were treated with DMSO (control) (A) or staurosporine (B), and then dually stained with TMRM and the green FLICA® poly caspase probe FAM-VAD-FMK (Cat. 92) and analyzed by flow cytometer. In the staurosporine-treated cells (B), there is loss of orange fluorescence (FL-2) and a concurrent increase in cell-bound green fluorescence (FL-1) due to caspase activation. Treatment with staurosporine induced apoptosis in about 90% of cells (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$305.60

ImmunoChemistry Technologies Tris Buffer Saline with Tween, 10X (TBST)

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ImmunoChemistry Technologies Tris-HCL Buffer, 10X

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ImmunoChemistry Technologies Tris-HCl Buffer, 10X, pH 10.0 Sodium Azide

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ImmunoChemistry Technologies Triton X100

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Biosensis TrkA-Fc Chimera, Human, Purified Recombinant Protein

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Biosensis TrkB (pS478) Positive Control Cell Lysate (Mouse NSC-34)

$177

Biosensis TrkB control peptide (54-67), Rat, Purified Peptide

$97

PhosphoSolutions TRPM7 Phospho-Regulation Antibody Kit

$522

ImmunoChemistry Technologies Trypsin Reagent

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ImmunoChemistry Technologies Tumor Necrosis Factor alpha

$699

ImmunoChemistry Technologies Tumor Necrosis Factor beta

$378.95

ImmunoChemistry Technologies Tween 20

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ImmunoChemistry Technologies UEA-I-Biotin, Conjugated

$204.40

ImmunoChemistry Technologies UEA-I-FITC, Conjugated

$204.40

Figure shows the two phosphate moieties of ADP, with no trace of a peak indicating the third phosphate of ATP using P31 NMR.

ImmunoChemistry Technologies Ultra Pure ADP

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ImmunoChemistry Technologies Universal Antibody Dilution Buffer,™ MIT-HCl

$65.30

ImmunoChemistry Technologies Universal Antibody Dilution Buffer™

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ImmunoChemistry Technologies Urease Conjugated with Biotin

$215.65

PhosphoSolutions Vav Phospho-Regulation Antibody Kit

$470

PhosphoSolutions VEGFR Phospho-Regulation Antibody Kit

$470

PhosphoSolutions WAVE Phospho-Regulation Antibody Kit

$470

ImmunoChemistry Technologies WGA-Biotin, Conjugated

$204.40

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