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Figure 2. Typical standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Fluorescent ADP Detection Kit

From $413.95

ImmunoChemistry Technologies Fluorescent ATP Detection Kit

From $413.95

Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.

Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.

ImmunoChemistry Technologies Fluorescent Cell Proliferation Assay

From $120.60

Antibodies Incorporated Fluorescent Goat anti-Human IgG for ANA kit In-Vitro Diagnostic Kit

$79

Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).

Figure 2. Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488 nm emission: 505-550 nm barrier filters. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.

ImmunoChemistry Technologies Fluorescent Hypochlorite Detection Kit

$533.50

ImmunoChemistry Technologies Fluorescent Lactate Detection Kit

$451.75

ImmunoChemistry Technologies Fluorescent Monoamine Oxidase Detection Kit

$304.55

Figure 2. Comparison of NADPH & NADH Standard Curves

ImmunoChemistry Technologies Fluorescent NADP/NADPH Detection Kit

From $622.45

Figure 2. Phosphate standard curve was generated using fluorometric detection: excitation 535 nm and emission 585 nm. Incubation time = 60 minutes at 37°C. Standard curve range 1.5625 μM to 100μM. Graph was plotted using 4PL non-linear regression.

ImmunoChemistry Technologies Fluorescent Phosphate Detection Kit

$284.10

ImmunoChemistry Technologies Fluorescent Sarcosine Detection Kit

$376.10

Figure 2. Bovine serum Semicarbazide-sensitive amine oxidase was serially diluted in 1X Reaction buffer. The serially diluted samples were run as described in the protocol. The samples were read after a 3 hours incubation period. Excitation: 530 nm and emission: 590 nm.

ImmunoChemistry Technologies Fluorescent Semicarbazide-Sensitive Amine Oxidase Detection Kit

$500.80

Figure 1. Standard curve (fluorescence). Excitation 535 nm and emission 585 nm. R2 value=0.9977. Incubation time= 1 hour at room temperature. Standard curve range 0.15625 μM to 10μM. A new curve must be generated each time the assay is run.

Figure 2. Standard curve (absorbance). Absorbance read-out at 570 nm. R2 =0.9993. Incubation time = 30 minutes. Standard curve range 1.25μM to 80μM. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Fluorescent Total Cholesterol Detection Kit

$376.10

ImmunoChemistry Technologies Fluoro AChE - a fluorescence assay for monitoring and detecting acetylcholinesterase activity in samples

From $512

Figure 2. The reaction contained 20mM hydrogen peroxide (final) per well and the indicated amounts of catalase. The reaction was incubated for 30 minutes at room temperature. Reaction cocktail was added to each well and the reaction incubated for another 10 minutes in the dark at room temperature. Fluorescence was measured at excitation 530nm and emission 590nm. The graphs show the net fluorescence change: Negative Control fluorescence (no catalase) minus Sample (Cat.alase) fluorescence.

ImmunoChemistry Technologies Fluoro Catalase Activity Detection Kit by Cell Technology

$359.75

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent, which is reduced by NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction (which does not react with NADP/NADPH).

Figure 2. NADH standard curve. Titrated in NADH standard diluent. Incubation time was 1 hour.

ImmunoChemistry Technologies Fluoro NAD/NADH Detection Kit by Cell Technology

From $622.45

Biosensis Fluoro-Jade B (FJB) Staining Kit

$597

Biosensis Fluoro-Jade C (FJC) Staining Kit

From $367

Biosensis Fluoro-Jade C (FJC) Staining Kit

$727

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing staining of GFAP. The sections were stained with Antibodies Incorporated anti-GFAP antibody (visualized in green) (Cat. 75-240) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.

Representative section of formalin fixed, paraffin-embedded rat brain showing staining of GFAP. The sections were stained with Aves Labs anti-GFAP antibody (visualized in green) (Cat. GFAP) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.

ImmunoChemistry Technologies Fluoroshield with DAPI & DABCO

From $102.20

ImmunoChemistry Technologies Fluoroshield with DAPI & PPD

From $102.20

ImmunoChemistry Technologies Fluoroshield with DAPI & Propylgallate

From $102.20

ImmunoChemistry Technologies Fluoroshield with Phenylenediamine

From $89.95

Distribution of α2δ-1 subunit in spinal dorsal horn. The spinal dorsal horn sections of CV mice were stained with anti-α2δ-1 antibody (green). Scale bar = 200 µm. Solid line represents the gray matter border, dotted line represents the superficial layer. Sections mounted with Fluoroshield medium (Cat. AR-6500, ImmunoBioScience Corp). Image from publication CC-BY-4.0. PMID: 38989140

ImmunoChemistry Technologies Fluoroshield™

From $89.95

The cell morphology of MDA-MB-231 breast cancer cells after they were treated with fludioxonil(Flu) for 72 h and were stained with DAPI and actin. The samples were sealed with mounting medium (Cat. AR-6515). Image from publication, CC-BY-4.0, PMID: 39201710.

ImmunoChemistry Technologies Fluoroshield™ with DABCO

From $89.95

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