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Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. MCF-7 cells were exposed to 0.15 µM camptothecin (Cat. 6210) for 24 hours at 37°C, then stained with MR-(DEVD)2 for 30 minutes at 37°C, washed, and then stained with 1 µg/mL Hoechst stain. Apoptotic cells with orange-red lysosomal bodies and less intense blue nuclei are intermixed with non-apoptotic cells bearing bright blue nuclei and absent or reduced orange-red lysosomal staining. Photo provided by Dr. Zbigniew Darzynkiewicz at Brander Cancer Research Center Institute, New York, NY.

ImmunoChemistry Technologies Magic Red® Fluorescent Caspase-3/7 Assay Kit

From $205.70

Figure 2. MCF-7 cells were stained with acridine orange for 30 minutes, then washed twice in PBS (cells not stained with Magic Red®) and then analyzed by fluorescence microscopy at 400X using either 492 nm excitation with a 540-550 nm emission filter (A, lysosomes appear yellowish green), or 540 nm excitation with a long pass >640 nm barrier filter (B, lysosomes appear red). Data from the laboratory of Dr. Z. Darzynkiewicz (Brander Cancer Research Center Institute, New York City, NY).

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin B Assay Kit

From $212.20

Figure 2. Intracellular cathepsin activity was detected in HL60 cells using Magic Red®-(LR)2 cathepsin K fluorogenic substrate. Intracellular localization of the hydrolyzed fluorescent Magic Red® product was detected using a Nikon Eclipse E800 photomicroscope equipped with a 510–560 nm excitation filter and a 570–620 nm emission/barrier filter at 500X (A). Photo at right (B) shows the corresponding DIC image of the cells. Data courtesy of Dr. Brian Lee, ICT, 061202.

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin K Assay Kit

From $212.20

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin L Assay Kit

From $212.20

PhosphoSolutions MAP Kinase Activation Antibody Kit

$459

PhosphoSolutions mDia Phospho-Regulation Antibody Kit

$459

PhosphoSolutions MeCP2 Phospho-Regulation Antibody Kit

$459

ImmunoChemistry Technologies Methyl Green

From $60

ImmunoChemistry Technologies Methylene Blue Counter Stain

From $60

PhosphoSolutions Microtubule Staining Kit

$459

Figure 1. Jurkat cells were stimulated with Staurosporine for 3 hours (B) or DMSO (A). Mito Flow reagent was added according to the protocol, incubated for 30 minutes and analyzed by flow cytometry: Ex:488nm Em: FL2 channel.

ImmunoChemistry Technologies Mito Flow - Mitochondrial membrane potential detection kit

From $288

Antibodies Incorporated Mitotic Spindle Positive Control

$67

ImmunoChemistry Technologies Monster Block ELISA Blocking Buffer

From $54

Antibodies Incorporated Mouse Anti-Human IgG Fc Secondary Antibody, Biotin

$217

ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG, Fc-HRP (Cat. 6101-HRP).

Antibodies Incorporated Mouse Anti-Human IgG Fc Secondary Antibody, HRP

$200

Human IgG was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc (Cat. 80-6101) followed by Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.

Antibodies Incorporated Mouse Anti-Human IgG Fc Secondary Antibody, Unconjugated

$169

Biosensis Mouse Beta-NGF Rapid ELISA Assay

From $417

Biosensis Mouse GDNF Rapid ELISA Assay

From $417

Biosensis Mouse mNGF/proNGF Combo Rapid ELISA Assay

$677

Biosensis Mouse Multi-Neurotrophin Screening Rapid ELISA Assay

$617

Biosensis Mouse NGFR/p75ECD Rapid ELISA Assay

From $417

Biosensis Mouse proNGF Rapid ELISA Assay

From $417

ImmunoChemistry Technologies Mouse Serum

$70

PhosphoSolutions Muscle Atrophy Ubiquitin Ligase Antibody Kit

$459

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