Anti-S100A5 Antibody (N176A/35)

Name: Anti-S100A5 Antibody
Brand: NeuroMab
SKU: 75-199
Price: 337.0 USD
Availability: InStock

Our mouse S100A5 monoclonal antibody from NeuroMab is produced in-house from clone N176/35. It detects mouse and rat S100A5 and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Immunoblot versus adult rat brain membranes (RBM) and whole brain homogenates from S100A5-tauGFP (targeted deletion of S100A5 and replaced with coding region of tau fused to GFP) heterozygote (Het) and KO mice and probed with N176A/35 TC supe (left) and rabbit anti-S100A5 antibody (right). Samples courtesy of Tim McClintock, University of Kentucky.

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-199

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

S100A5

Scientific Background

Protein S100A5 is encoded by the gene S100A5 and a member of the S100 family of proteins. S100A5 is a calcium-binding cytosolic proteins and is also a member of the damage-associated molecular pattern molecules (DAMPs) family, a group of proteins that are released from the extracellular or intracellular space following tissue injury or cell death. S100A5 is localized in the cytoplasm and implicated in the regulation of a number of cellular processes such as cell cycle progression and extracellular signal transduction. S100A5 has been found in brain and kidney. Diseases associated with this gene include Astrocytoma.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N176A/35

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

S100a5 S100d

Molecular Weight

10 kDa

Immunogen

Fusion protein amino acids 1-93 (full-length) of rat S100A (accession number P63083) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315917

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with S100A4 or S100A6 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Protein S100-A5 (Protein S-100D) (S100 calcium-binding protein A5)

UniProt ID

P63083

Shipping

Shipped on ice packs

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Anti-S100A5 Antibody (N176A/35)

SKU: 75-199

Product Details

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