Our Anti-Rhodopsin primary antibody from PhosphoSolutions is mouse monoclonal. It detects amphibians and most mammals Rhodopsin and is Protein G purified. It is great for use in WB, IHC.
Immunofluorescence of mouse retinal section showing specific immunolabeling of the rhodopsin protein (Cat. 1840-RHO, red, 1:100) in the rod spherules. Photo courtesy Mary Raven, University of California, Santa Barbara, CA.
Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.
Protein G Purified
Monoclonal
1D4
IgG1
ELISA, IHC, WB
Mouse
RHO
39 kDa
Purified native bovine rhodopsin.
Bovine
AB_2492232
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:4000
IHC: 1:100-1:200
Unconjugated
Specific for endogenous levels of the ~39 kDa rhodopsin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
