Anti-RGS14 Antibody (N133/21)

Our mouse RGS14 monoclonal antibody from NeuroMab is produced in-house from clone N133/21. It detects human, mouse, and rat RGS14 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunoblot against lysates from wild-type (+/+) and RGS14 knockout (-/-) mouse brains probed with N133/21. Data courtesy of John Hepler (Emory).
Coronal section from a C57BL/6J mouse showing the hippocampus co-stainedwith RGS14 (Cat. 75-170, 1:500; pink) and CaMKII alpha (green). The merged image is shown on the right. Image from publication CC-BY-4.0. PMID:36971428
Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.
Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N133/21 (left) or N52A/42 (right) TC supe.
Representative images of RGS14 and PCP4 immunofluorescence. Colored lines indicate the RGS14+/PCP4+ double-labeled zone (DLZ) in yellow, with RGS14 (Cat. 75-170, 1:500; red) and PCP4 (green) extension beyond the DLZ, in dorsal (b) and ventral (c) depths of mouse hippocampus. Image from publication CC-BY-4.0. PMID:36762588
i. Confocal image of mouse CA2 immunolabeled with RGS14 (ca. 75-170, 1:500; green) and SUM afferent marker VGLUT2 (magenta). j Confocal image from the CA2 immunolabeled with RGS14 (ca. 75-170, 1:500; green) and cholinergic afferent marker VAChT (magenta). Image from publication CC-BY-4.0. PMID:36991001
Coronal section from a C57BL/6J mouse CA2 hippocampus showing duolink staining where signal (red) denotes interaction between two proteins targeted with primary antibodies RGS14 (Cat. 75-170, 1:500) and CaMKII to test for protein interaction. Image from publication CC-BY-4.0. PMID:36971428
High magnification view of the central amygdala (CeA) ipsilateral to the insula with an AAV injection in mouse. Left: GFP, green(axon arbors restricted to the lateral and capsular divisions of the CeA (CeL/CeC)), middle: anti-RGS14 (Cat. 75-170, 1:500; magenta) immunostaining identifying the CeL/CeC but not medial division of the CeA (CeM), right: merged. Image from publication CC-BY-4.0. PMID:37378339
Representative confocal serial sections of mouse hippocampus infected with M1-EGFP (green) and stained with RGS14 (75-170, 1:500; red). B5 Magnified image of M1-EGFP (green) collocated with RGS14 (red) in CA2 region. Image from publication CC-BY-4.0. PMID:37303064
Representative confocal serial sections of hippocampus infected with AAV9/hSyn-Cre & AAV9/EF1α-DIO-EGFP (green) in dCA2 and stained with RGS14 (75-170, 1:500; red). D5 Magnified image of hSyn driven EGFP (green) and RGS14 (75-170, 1:500; red). Image showing no hSyn-EGFP cells and RGS14 overlapping, and EGFP expressed within CA2. Image from publication CC-BY-4.0. PMID:37303064
Representative images and quantification of PV+ puncta density in mice CA2/CA3 stratum pyramidale. Top: RGS14+ labeling (Cat. 75-170, 1:400) defines CA2 subfield. Image from publication CC-BY-4.0. PMID:37797581
Representative images of GFP expression (GFP) and anti-RGS14 (Cat. 75-170) labeling to localize CA2 from CA3 and CA1 (dotted lines) in naive rat brain. Image from publication CC-BY-4.0. PMID:37833914
Immunoblot against lysates from wild-type (+/+) and RGS14 knockout (-/-) mouse brains probed with N133/21. Data courtesy of John Hepler (Emory).
Immunoblot against lysates from wild-type (+/+) and RGS14 knockout (-/-) mouse brains probed with N133/21. Data courtesy of John Hepler (Emory).
Click on image to zoom

Human, Mouse, Rat
ELISA, ICC, IHC, WB
Mouse
490
550
594
650
Biotin
Carrier-Free

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Data Sheet
KO Validated KO Validated Trial Size Trial Size

SKU: 75-170

Volume: 100 µL
Price:
$394.00
Ships: 1-2 business days

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