Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

Name: Anti-PSD-95 MAGUK Scaffold Protein Antibody
Brand: NeuroMab
SKU: 75-348
Price: 394.0 USD
Availability: InStock

Our mouse PSD-95 MAGUK scaffold protein monoclonal antibody from NeuroMab is produced in-house from clone K28/74. It detects human, mouse, non-human primate, and rat PSD-95 MAGUK scaffold Protein And is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, ELISA, AT.

Immunofluorescence of human frontal cortex with K28/74 (green), rabbit Synapsin-1 (red) and DAPI (blue). Twelve images from 100 nm sections (left) were volume-reconstructed via array tomography (31.5 x 34 x 1.2 mm, right). Image courtesy of Nancy O’Rourke, Nafisa Ghori and Stephen Smith (Stanford).

Western blot showing specific labeling of PSD95 (Cat. 75-348) in wild type (WT) and Mir324 KO mouse hippocampus. Image from publication CC-BY-4.0. PMID:38082035

Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD- 95 or PSD-93 knockout (KO) mice probed with K28/74 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).

Immunofluorescence of human frontal cortex with K28/74 (green), rabbit Synapsin-1 (red) and DAPI (blue). Twelve images from 100 nm sections (left) were volume-reconstructed via array tomography (31.5 x 34 x 1.2 mm, right). Image courtesy of Nancy O’Rourke, Nafisa Ghori and Stephen Smith (Stanford).

Click on image to zoom

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-348

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

PSD-95 MAGUK scaffold protein

Scientific Background

PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K28/74

Isotype

IgG1

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

DLG4 PSD95

Molecular Weight

95-110 kDa (varies with cell background due to phosphorylation)

Immunogen

Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2315909

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with DLG1/SAP97

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

UniProt ID

P78352

Shipping

Shipped on ice packs

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Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

SKU: 75-348

Product Details

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