Anti-PSD-95 Antibody (K28/43)

Our mouse PSD 95 monoclonal antibody from NeuroMab is produced in-house from clone K28/43. It detects chicken, human, mouse, non-human primate, and rat PSD 95 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA, EM.

Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2).
Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD-95 and -93 knockout (KO) mice probed with K28/43 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, Howard Hughes Medical Institute).
Immunolabelling of fixed primary rat hippocampal neurons for SENP1 and PSD95. Image from publication CC-BY-4.0. PMID: 35739402
Western blot of total homogenate (H) and TIF fraction from rat hippocampal neurons treated with vehicle (CTRL) or NMDA. Image from publication CC-BY-4.0. PMID: 35613587
Electron micrograph of K28/43 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) are seen in the postsynaptic densities of dendritic spines (s) forming asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
Adult rat brain immunohistochemistry.
Double staining of GluA1 (red) and PSD-95 (green) in P12 MNTB neurons of control (left) and Nlgn3 KO (right) mice. Image from publication CC-BY-4.0. PMID: 35704570
Western blot analysis of PSD-95 and synaptophysin protein in the hippocampus region of AD mice. Image from publication CC-BY-4.0. PMID: 35701825
Expression levels of ARID1B protein in WT mouse brains at E18, P0, P7, P14, P21, P28, P35, and P42, as determined by immunoblot analysis of whole-brain lysates. PSD-95, an abundant excitatory postsynaptic density protein, is shown for comparison. The full-length blot was divided into three parts for immunoblotting with different antibodies. Similar results were obtained from three independent experiments. Image from publication CC-BY-4.0. PMID: 36030255
Immunolabeling of Synapsin 1 (SYN1) and PSD95-positive cells (green and red respectively) within MAP2+ neurons (white) in ight-week-old human brain cortical organoids (BCO) infected at MOI 2.5 and treated with vehicle (Veh) or 20μM Sofosbuvir (SOF). Image from publication CC-BY-4.0. PMID:36327326
Representative images of HEK293 cells transfected with different combinations of PSD95 (Cat. 75-028, 1:2000), GluN2A-CTD, WT, and R9A Rph3A. Image from publication CC-BY-4.0. PMID:36693856
Primary cultures of rat hippocampal neurons were treated at DIV9 with either 2 μg/μl sOligo or PBS and analyses were performed at DIV16. Scaffolding protein Rph3A and PSD-95 (Cat. 75-028, 1:1000) were evaluated by Western blot in TIF from sOligo- or PBS-neurons. Image from publication CC-BY-4.0. PMID:37009450
Representative confocal images of mouse cortical neurons using DAPI, MAP2, and PSD95 (Cat. 75-028, 1:1000) in control conditions (upper images) and treated with DAPT inhibitor (lower images). Image from publication CC-BY-4.0. PMID:37059474
Effects of Ank2 cKO on the localization of Kv7.3 at excitatory and inhibitory synapses in cultured mouse cortical neurons (DIV 17). Note that the colocalization, determined by colocalized areas, between Kv7.3 (green) and PSD-95 (Cat. 75-028, 1:1000; red) (an excitatory synapse marker) is not changed, whereas the mean intensity of Kv7.3 at the colocalized area is moderately decreased. Image from publication CC-BY-4.0. PMID:37321992
Immunostaining of Cas9-expressing cortical neuronal cultures transduced with mock lentiviral vectors (control, left), lentiviral vectors carrying gRNAs to delete Pcdh15 (Pcdh15 Δex6, middle), and sPcdh15-expressing vectors (sPcdh15, right) with the neuronal markers PSD95 (Cat. 75-028) and MAP2. Upper: PSD95-expressing puncta. Middle: overlay of PSD95 and MAP2. Lower: magnification of middle panel. Image from publication CC-BY-4.0. PMID:37595869
Dissociated hippocampal neurons isolated from P0 Emx1Cre:R26P1 and Emx1Cre;R26P1R203W mice were processed for confocal imaging at DIV18 to detect MAP2 (magenta), HA-tagged PACS1 or PACS1R203W (pseudocolored green), PSD95 (Cat. 75-028, 1:250; pseudocolored red) and DAPI (blue). Image from publication CC-BY-4.0. PMID:37848409
DIV18 neurons prepared from WT C57BL/6 mice were processed for confocal imaging to detect endogenous HDAC6 (green), PACS1 (red), and PSD95 (Cat. 75-028, 1:250; blue), and the fluorescent signal from the indicated spine (yellow line) was measured (Nikon Elements). Image from publication CC-BY-4.0. PMID:37848409
Immunofluorescences against PSD95 cat. 75-028) and Synaptophysin in wild-type, FL, and FL1-injected mouse neurons. Image from publication CC-BY-4.0. PMID:37957716
Single confocal images of wildtype (top), heterozygote (middle) and mutant (bottom), showing the colocalization of vGlut1+ boutons (blue) and PSD95+ clusters (Cat. 75-028) onto VIP+ proximal dendrites (white outline) (yellow arrows) at P45. Image from publication CC-BY-4.0. PMID:38201256
Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2).
Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2).
Click on image to zoom

Chicken, Human, Mouse, Non-Human Primate, Rat
ELISA, EM, ICC, IHC, IP, WB
Mouse
490
550
594
650
Biotin
Carrier-Free

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Data Sheet
KO Validated KO Validated Trial Size Trial Size

SKU: 75-028

Volume: 100 µL
Price:
$394.00
Ships: 1-2 business days

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