Anti-Pregnancy Associated Plasma Protein A (PAPP-A) Antibody (9)
Our mouse Pregnancy Associated Plasma Protein A (PAPP-A) monoclonal antibody from Antibodies Incorporated is produced in-house from clone 9. It detects human Pregnancy Associated Plasma Protein A (PAPP-A) and is Protein A or Protein G purified. Works in ELISA, IHC, WB.
Immunohistochemistry detection of Pregnancy associated plasma protein A (PAPP-A) in human placenta tissue using Anti-Pregnancy Associated Plasma Protein A (PAPP-A) Antibody (Cat. BTE 004-09-010). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. BTE 004-09-010) was incubated with the sample. Staining is restricted to the syncytiotrophoblast of the chorionic villi.
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SKU: BTE 004-09-010
Ships: 1-2 business days
Product Details
Pregnancy Associated Plasma Protein A (PAPP-A)
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
9
IgG2b/k
ELISA, IHC, WB
Mouse
PAPPA
500 kDa (tetramer), 180 kDa (monomer)
Purified human PAPP-A
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
In sandwich ELISA the optimal combination is BTE 004-09 used as coating antibody and biotinylated ABS 006-24 used as a detection antibody. BTE 004-09 can also be used as a detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. BTE 004-09 detect both reduced and unreduced PAPP-A in Western Blotting, though the reaction to unreduced is less than that of ABS 006-01 and ABS 006-24.
Unconjugated
BTE 004-09 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A. The epitope of BTE 004-09 is different from ABS 006-01 and ABS 006-24. The epitope is located on the PAPP-A subunit of the heterotetramer.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
PAPPA, ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A, PAPPA1, pappalysin-1, IGF-dependent IGFBP-4 protease, aspecific BCL2 ARE-binding protein 2, differentially placenta 1 expressed protein, insulin-like growth factor-dependent IGF binding protein-4 protease, pregnancy-associated plasma protein A, pappalysin 1
5069
Shipped on ice packs

