Anti-Phosphoserine/threonine Antibody (M380A/M380B)

Our Anti-Phosphoserine/threonine mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is Protein A purified. It is great for use in WB, ICC, IP.

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine (PM3801) and rabbit polyclonal anti-Phosphoserine/threonine (PP2551), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Western blot showing phosphorylation level (Cat. PM3801, 1:2000) of NFATC1 in TAZ overexpressed HEK-293T cells after 1 mM Tg treatment or UCHL1 knockout. Image from publication CC-BY-4.0. PMID: 37215988
WT (PKY2255) and pda1Δ (PKY2256) cells expressing Qcr2-GFP were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. The immunoprecipitates were then probed with α- phosphoamino acid antibody (Cat. PM3801, top panel) and α-GFP antibody (bottom panel). Right panel: day 5 samples were treated overnight with lambda phosphatase or mock-treated. Image from publication CC-BY-4.0. PMID: 37454238
WT (PKY1089) and pda1Δ (PKY1093) cells expressing Mdh1-GFP, as well as the indicated plasmids, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody (Cat. PM3801), and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector Image from publication CC-BY-4.0. PMID: 37454238
Mutant lat1Δ cells (PKY2298) expressing the indicated plasmids, as well as Mdh1-GFP, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody (Cat. PM3801), and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector. Image from publication CC-BY-4.0. PMID: 37454238
In vitro kinase assay of nrdD stained with a serine/threonine site-specific antibody (Cat. PM3801). Image from publication CC-BY-4.0. PMID: 38683735
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
Click on image to zoom

Human, Mouse, Rat
ELISA, ICC, IP, WB
Mouse

Go to Full Product Details

Data Sheet

SKU: PM3801

Volume: 100 µL
Price:
$348.00
Ships: 1-2 business days

Bulk Order Anti-Phosphoserine/threonine Antibody

Related Products

Recently Viewed