Anti-Phospho-GUK1 (Tyr74) Antibody

Our GUK1 (Tyr74) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat GUK1 (Tyr74) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, ELISA.

Western blot detection of GUK1 phosphorylated at Tyr74 using anti-pGUK1 Tyr74 rabbit polyclonal antibody (Cat No. pGUK1-74 ). Whole cell lysates were generated from HEK293T cells stably co-expressing EML4-ALK fusion protein with FLAG-GUK1-WT or FLAG-GUK1-Y74F mutant, run on SDS-PAGE gel, transferred to a nitrocellulose membrane and examined by Western blot. The blot was probed with pGUK1-Y74 primary antibody at a concentration of 1:200 and then detected via chemiluminescence using a Goat Anti-Rabbit H+L, HRP conjugated secondary antibody. Actin was used as a loading control to show equal loading of both lysates. pGUK1 Y74 antibody recognizes only GUK1-WT and not the phospho-deficient mutant GUK1-Y74F at the expected molecular weight of ~25 kDa. Image courtesy of Kiran Kurmi, University of Miami Miller School of Medicine.
Western blot detection of GUK1 phosphorylated at Tyr74 using anti-pGUK1 Tyr74 rabbit polyclonal antibody (Cat No. pGUK1-74 ). Whole cell lysates were generated from HEK293T cells stably co-expressing EML4-ALK fusion protein with FLAG-GUK1-WT or FLAG-GUK1-Y74F mutant, run on SDS-PAGE gel, transferred to a nitrocellulose membrane and examined by Western blot. The blot was probed with pGUK1-Y74 primary antibody at a concentration of 1:200 and then detected via chemiluminescence using a Goat Anti-Rabbit H+L, HRP conjugated secondary antibody. Actin was used as a loading control to show equal loading of both lysates. pGUK1 Y74 antibody recognizes only GUK1-WT and not the phospho-deficient mutant GUK1-Y74F at the expected molecular weight of ~25 kDa. Image courtesy of Kiran Kurmi, University of Miami Miller School of Medicine.
Western blot detection of GUK1 phosphorylated at Tyr74 using anti-pGUK1 Tyr74 rabbit polyclonal antibody (Cat No. pGUK1-74 ). Whole cell lysates were generated from HEK293T cells stably co-expressing EML4-ALK fusion protein with FLAG-GUK1-WT or FLAG-GUK1-Y74F mutant, run on SDS-PAGE gel, transferred to a nitrocellulose membrane and examined by Western blot. The blot was probed with pGUK1-Y74 primary antibody at a concentration of 1:200 and then detected via chemiluminescence using a Goat Anti-Rabbit H+L, HRP conjugated secondary antibody. Actin was used as a loading control to show equal loading of both lysates. pGUK1 Y74 antibody recognizes only GUK1-WT and not the phospho-deficient mutant GUK1-Y74F at the expected molecular weight of ~25 kDa. Image courtesy of Kiran Kurmi, University of Miami Miller School of Medicine.
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Human, Mouse, Rat
ELISA, ICC, IHC, WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: pGUK1-74

Volume: 100 µL
Price:
$456.00
Ships: 1-2 business days

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