Anti-Peptide Histidine Methionine Antibody (3)
Our mouse Peptide Histidine Methionine monoclonal antibody from Antibodies Incorporated is produced in-house from clone 3. It detects human Peptide Histidine Methionine and is Protein A or Protein G purified. Works in ELISA, IHC.
Immunohistochemistry detection of Peptide Histidine Methionine in human appendix tissue using Anti-Peptide Histidine Methionine Antibody (Cat. ABS 020-03-010). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. ABS 020-03-010) was incubated with the sample at a dilution of 1:200. Plasma of the veins stained strongly as well as ganglion cells of neuronal plexuses and neuronal axons.
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SKU: ABS 020-03-010
Ships: 1-2 business days
Product Details
Peptide Histidine Methionine
Human peptide histidine-methionine (PHM) is a 27 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. PHM is produced from the same prohormone as VIP, with which it shows considerable sequence homology. The VIP/PHM prohormone is produced by neurons in the central and peripheral nervous systems, often together with acetylcholine. In some situations the effect of the peptides is to reinforce and prolong the effects of neurotransmitter release. Like VIP, PHM is a vasodilator and stimulator of intestinal fluid secretion. High plasma levels of PHM are diagnostic of the corresponding peptide-producing tumor (VIPoma), associated with the watery diarrhea-hypokalemia-achlorhydria syndrome.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
3
IgG2a/k
ELISA, IHC
Mouse
VIP
3 kDa
Synthetic human peptide histidine-methionine
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
IHC: 1:200
ELISA: 1:2000
ELISA: 1:2000
ABS 020-03 binds free PHM in solution. An ELISA dilution guideline of 1/2000 applies to PHM coated directly at 2 μg/mL. For glutaraldehyde-linked PHM-ovalbumin (molar ratio 4:1) coated at 1 μg/mL the dilution is 1/30,000. ABS 020-03 was used in IHC on a human appendix section.
Unconjugated
ABS 020-03 binds human PHM and cross-reacts with PACAP (approximately 12%; incomplete, non-parallel inhibition). ABS 020-03 does not cross-react with GLP-1, GLP-2, VIP or glucagon.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
VIP, PHM27, VIP peptides, prepro-VIP
7432
Shipped on ice packs

