Anti-Pan-Gamma-Protocadherin-A Antibody (N144/32)

Name: Anti-Pan-Gamma-Protocadherin-A Antibody
Brand: NeuroMab
SKU: 75-178
Price: 337.0 USD
Availability: InStock

Our mouse Pan-Gamma-protocadherin-A monoclonal antibody from NeuroMab is produced in-house from clone N144/32. It detects human, mouse, and rat Pan-Gamma-protocadherin-A and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Immunoblot versus brain samples from WT and Gamma- protocadherin KO mice. Data courtesy of Joshua Weiner, University of Iowa.

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma protocadherin-A3 and -A4 and untagged Kv2.1 plasmids and probed with N144/32 (left) and N159/5 (right) TC supe.

Immunoblot versus brain samples from WT and Gamma- protocadherin KO mice. Data courtesy of Joshua Weiner, University of Iowa.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-178

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Pan-Gamma-protocadherin-A

Scientific Background

Cadherins are calcium-dependent cell-cell adhesion molecules, and protocadherins constitute a subfamily of non-classic cadherins. Members of the protocadherin gamma subfamily are encoded by 22 tandemly arranged genes within the PCDHG gene cluster on chromosome 5q31. The 22 PCDHG genes function as 'variable' exons that are individually spliced to a downstream constant region to form distinct PCDHG transcripts. The variable PCDHG exons encode the extracellular and transmembrane domains of the protocadherin protein, and the common region encodes the intracellular domain. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N144/32

Isotype

IgG2b

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

Pcdhga3

Molecular Weight

100 kDa

Immunogen

Fusion protein corresponding to amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-A3 (accession number Q91XY5) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2159447

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.106

Conjugate

Unconjugated

Specificity

Cross-reacts with all Gamma protocadherin-A, -B and -C proteins

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Protocadherin gamma A3 (Protocadherin gamma subfamily A, 3)

UniProt ID

Q91XY5

Shipping

Shipped on ice packs

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Anti-Pan-Gamma-Protocadherin-A Antibody (N144/32)

SKU: 75-178

Product Details

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