Anti-Olig1 Recombinant Chicken Chimeric mAb (N149/25)

Name: Anti-Olig1 Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-180
Price: 481.0 USD
Availability: InStock

Our chicken Anti-Olig1 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N149/25. It detects mouse and rat Olig1 and is purified by affinity chromatography. Works in WB, IHC, ICC, ELISA.

Representative image of 4% paraformaldehyde fixed free-floating rat brain sections stained with anti-Olig1 recombinant chicken monoclonal antibody (red) at 1:1000 dilution and co-stained with anti-MAP2 rabbit polyclonal antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-Olig1 specifically stains the oligodendrocytes. Image courtesy of EnCor Biotechnology.

Formalin fixed, paraffin-embedded rat brain hippocampus showing oligodendocytes stained by Olig1 recombinant chicken monoclonal antibody (Cat. 78-180) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). Mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Olig1 specifically stains the oligodendrocytes present in the CA3 and CA4 regions of the hippocampus.

Western blotting of mock transfected and Olig1 transfected COS-7 lysates with anti-Olig1 mouse NeuroMab (Cat. 75-180) and Aves Labs anti-Olig1 recombinant chicken Mab (Cat. 78-180) antibody. The mouse mAb and chicken mAb were detected with anti-mouse-HRP and anti-chicken-HRP (Cat. 80-1000-HRP), respectively. Aves Labs Recombinant Chicken anti-Olig1 mAb recognizes exogenous Olig1 with similar sensitivity as the anti-Olig1 NeuroMab only in Olig1 transfected COS-7 lysates at ~30 kDa.

Formalin fixed, paraffin-embedded rat brain cerebral cortex showing oligodendocytes stained by Olig1 recombinant chicken monoclonal antibody (Cat. 78-180) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Olig1 specifically stains the oligodendrocytes in the cerebral cortex.

Representative image of 4% paraformaldehyde fixed free-floating rat brain sections stained with anti-Olig1 recombinant chicken monoclonal antibody (red) at 1:1000 dilution and co-stained with anti-MAP2 rabbit polyclonal antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-Olig1 specifically stains the oligodendrocytes. Image courtesy of EnCor Biotechnology.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

Trial Size

SKU: 78-180

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Olig1

Scientific Background

Oligodendrocyte transcription factor 1, Basic Domain, Helix-Loop-Helix Protein, Class B, 6 or Class E Basic Helix-Loop-Helix Protein 21 is encoded by the gene Olig1. Olig1 is a transcription factor and a member of the basic helix-loop-helix (bHLH) factor family. Olig1 promotes formation and maturation of oligodendrocytes (oligodendrogensis). Olig1 is expressed in spinal cord and brain during development, and by oligodendrocytes after late embryonic development. Olig1 is a useful marker for Oligodendrocyte staining. Diseases associated with this gene include Oligodendroglioma and Grade Iii Astrocytoma.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N149/25

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Olig1

Molecular Weight

30 kDa

Immunogen

Fusion protein amino acids 1-67 (N-terminus) of mouse Olig1 (accession number Q9JKN5) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N149/25 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N149/25 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

No cross-reactivity against Olig2

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Oligodendrocyte transcription factor 1 (Oligo1) (Oligodendrocyte-specific bHLH transcription factor 1)

UniProt ID

Q9JKN5

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Olig1 Recombinant Chicken Chimeric mAb (N149/25)

SKU: 78-180

Product Details

Bulk Order Anti-Olig1 Recombinant Chicken Chimeric mAb

Related Products

Recently Viewed