Anti-NFM (Neurofilament Medium Chain) Antibody

NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.

Immunocytochemical staining of NF-M immunoreactivity (green) and Glial Fibrillary Acidic Protein (GFAP, red) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.
Immunocytochemical staining of NF-M immunoreactivity (green) and Glial Fibrillary Acidic Protein (GFAP, red) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.
Immunocytochemical staining of NF-M immunoreactivity (green) and Glial Fibrillary Acidic Protein (GFAP, red) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.
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Human, Mouse, Rat
ICC, IHC, WB
Chicken

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Data Sheet

SKU: NFM

Volume: 200 µL
Price:
$301.00
Ships: 1-2 business days

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