Anti-mScarlet3 Antibody

Name: Anti-mScarlet3 Antibody
Brand: Aves Labs
SKU: MSCARLET3-0100
Price: 481.0 USD
Availability: InStock

Our Anti-mScarlet3 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a mScarlet3, and is affinity-purified IgY. Works in ICC, IHC, WB.

Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.

Western blotting of mock or mScarlet3 transfected COS-7 cell lysates with chicken anti-mScarlet3 antibody (0.5 ug/ml) and detected with anti-chicken HRP. Chicken anti-mScarlet3 recognizes exogenous mScarlet3 in COS-7 cells.

Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.

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Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: MSCARLET3-0100

Volume: 500 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

mScarlet3

Scientific Background

mScarlet3 is a next-generation red fluorescent protein (RFP) widely used in cellular and molecular biology for live-cell imaging. It is part of the mScarlet series, developed to improve brightness, photostability, tolerance to acidity, and maturation speed over previous RFPs (Bindels et al 2017). mScarlet3 introduces enhancements like better acid tolerance and increased brightness, enabling the capture of clearer, precise, long-term images of live cells. These improvements are especially valuable in studying dynamic cellular processes, such as protein expression and cell signaling where prolonged and high-resolution imaging is essential. mScarlet3 enables the tracking of protein expression, cell signaling, and subcellular dynamics with greater fidelity and less cellular stress.

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

26 kDa

Immunogen

Purified recombinant mScarlet3 expressed in E.Coli

Immunogen Species

Human

Antibody Registry ID

AB_3665368

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
ICC: 1:50-1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

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Anti-mScarlet3 Antibody

SKU: MSCARLET3-0100

Product Details

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