Anti-Microtubule-Associated Protein (MAP2) Antibody

Name: Anti-Microtubule-Associated Protein (MAP2) Antibody
Brand: Aves Labs
SKU: MAP
Price: 481.0 USD
Availability: InStock

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P20357) and human (NP_002365) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain (1:1000 dilution, green); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs Cat No. F-1005, 1:500 dilution). Blue staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)

Neonatal mouse brain cortical cultures were stained with Aves Labs MAP2 (green) and GFAP (red, rabbit antibody); Blue is DAPI nuclear stain. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

Western blot analysis of MAP2 (Cat. MAP) in adult mouse cerebral cortex showing significant reduction in the integrated density of MAP2 protein bands normalized with GAPDH. Image from publication CC-BY-4.0. PMID:37416774

Immunostaining of control and Emx1Cre;CasTcKO coronal sections for MZ and SP (white arrows) markers at E15.5. MAP2 (Cat. MAP, 1:500), CSPG, Calretinin, and Calbindin show clustering of cells (yellow arrows). CSPG+ cells form a column connecting the SP with the MZ (yellow arrow). Thalamocortical afferents labeled with Calretinin show ectopic bundles extending from the IZ (white arrowheads). DAPI was used as a counterstain (blue). Image from publication CC-BY-4.0. PMID:37540708

MAP2 (Cat. MAP) immunostaining of neonatal (P1) mouse cerebral cortex showing a significant decrease in the area ratio of MAP2 fluorescence. Image from publication CC-BY-4.0. PMID:37416774

Representative images (E) and quantification (F) of MAP2 (Cat. MAP, red) immunofluorescence staining in xenografts dissected from nude mice harbouring 3117-shCtrl or 3117-shDKK4 intraperitoneally. Image from publication CC-BY-4.0. PMID:38519641

MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain (1:1000 dilution, green); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs Cat No. F-1005, 1:500 dilution). Blue staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Go to Full Product Details

Data Sheet

Trial Size

SKU: MAP

Volume: 1000 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Microtubule-Associated Protein (MAP2)

Scientific Background

Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape.

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MAP2

Molecular Weight

280 kDa

Immunogen

Synthetic peptides

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313549

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

Microtubule-associated protein 2 (MAP-2)

UniProt ID

P11137

Shipping

Ice packs

Recover password

Create my account

Anti-Microtubule-Associated Protein (MAP2) Antibody

SKU: MAP

Product Details

Bulk Order Anti-Microtubule-Associated Protein (MAP2) Antibody

Related Products

Recently Viewed