Anti-Microtubule Associated Protein 2 (MAP2) Antibody

Name: Anti-Microtubule Associated Protein 2 (MAP2) Antibody
Brand: PhosphoSolutions
SKU: 1099-MAP2
Price: 456.0 USD
Availability: InStock

Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from PhosphoSolutions is goat polyclonal. It detects human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (Cat. 1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (Cat. 1120-MBP , 1:5000, green). The blue is DAPI revealing nuclear DNA.

Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Immunofluorescent image of primary mouse neurons specifically labelling MAP2 (Cat. 1099-MAP2, 1:1000, red). The blue nuclear stain is Hoechst. The image was kindly provided by Noah Johnson, University of Colorado, Anschutz Medical Campus.

Immunolabeling for GSK3β (green) and MAP2 (Cat. 1099-MAP2; magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. Image from publication CC-BY-4.0. PMID: 37919281

Immunolabeling of REST (green), the neuron marker MAP2 (Cat. 1099-MAP2; magenta) and nuclei (DAPI, blue) in the CA1 region of the hippocampus, shows elevated nuclear REST levels in 3-month-old J20 mice. Image from publication CC-BY-4.0. PMID: 37919281

Immunolabeling of REST (green) and neuron marker MAP2 (Cat. 1099-MAP2; red) in 3xTg primary cortical neurons (PCNs) shows nuclear REST in neurons treated with vehicle and decreased nuclear REST in 3xTg neurons after a 24-h treatment with the Wnt/β-catenin inhibitors Dickkopf 1 (DKK1), XAV939 and ICG001, or the PERK inhibitor GSK2606414. Image from publication CC-BY-4.0. PMID: 37919281

Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (Cat. 1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (Cat. 1120-MBP , 1:5000, green). The blue is DAPI revealing nuclear DNA.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Goat

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Data Sheet

Trial Size

SKU: 1099-MAP2

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Microtubule Associated Protein 2 (MAP2)

Scientific Background

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).

Format

Protein G Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Goat

Gene Name

MAP2

Molecular Weight

280 kDa

Immunogen

Recombinant human MAP2 protein expressed in and purified from E. Coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2752241

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified

Buffer

PBS + 50% glycerol and 5 mM NaN3

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~280 kDa MAP2 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody

UniProt ID

P11137

NCBI Gene ID

4133

Shipping

Blue Ice

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Anti-Microtubule Associated Protein 2 (MAP2) Antibody

SKU: 1099-MAP2

Product Details

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